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Open AccessArticle

Altered Expression of GABAergic Markers in the Forebrain of Young and Adult Engrailed-2 Knockout Mice

1
Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, 38068 Rovereto, Italy
2
Center for Mind/Brain Sciences (CIMeC), University of Trento, 38068 Rovereto, Italy
3
C.N.R. Neuroscience Institute, 5624 Pisa, Italy
*
Authors to whom correspondence should be addressed.
Genes 2020, 11(4), 384; https://doi.org/10.3390/genes11040384
Received: 23 February 2020 / Revised: 25 March 2020 / Accepted: 30 March 2020 / Published: 1 April 2020
Impaired function of GABAergic interneurons, and the subsequent alteration of excitation/inhibition balance, is thought to contribute to autism spectrum disorders (ASD). Altered numbers of GABAergic interneurons and reduced expression of GABA receptors has been detected in the brain of ASD subjects and mouse models of ASD. We previously showed a reduced expression of GABAergic interneuron markers parvalbumin (PV) and somatostatin (SST) in the forebrain of adult mice lacking the Engrailed2 gene (En2-/- mice). Here, we extended this analysis to postnatal day (P) 30 by using in situ hybridization, immunohistochemistry, and quantitative RT-PCR to study the expression of GABAergic interneuron markers in the hippocampus and somatosensory cortex of En2-/- and wild type (WT) mice. In addition, GABA receptor subunit mRNA expression was investigated by quantitative RT-PCR in the same brain regions of P30 and adult En2-/- and WT mice. As observed in adult animals, PV and SST expression was decreased in En2-/- forebrain of P30 mice. The expression of GABA receptor subunits (including the ASD-relevant Gabrb3) was also altered in young and adult En2-/- forebrain. Our results suggest that GABAergic neurotransmission deficits are already evident at P30, confirming that neurodevelopmental defects of GABAergic interneurons occur in the En2 mouse model of ASD. View Full-Text
Keywords: interneuron; parvalbumin; somatostatin; GABA; receptor; hippocampus; somatosensory; autism; mouse; development interneuron; parvalbumin; somatostatin; GABA; receptor; hippocampus; somatosensory; autism; mouse; development
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MDPI and ACS Style

Provenzano, G.; Gilardoni, A.; Maggia, M.; Pernigo, M.; Sgadò, P.; Casarosa, S.; Bozzi, Y. Altered Expression of GABAergic Markers in the Forebrain of Young and Adult Engrailed-2 Knockout Mice. Genes 2020, 11, 384. https://doi.org/10.3390/genes11040384

AMA Style

Provenzano G, Gilardoni A, Maggia M, Pernigo M, Sgadò P, Casarosa S, Bozzi Y. Altered Expression of GABAergic Markers in the Forebrain of Young and Adult Engrailed-2 Knockout Mice. Genes. 2020; 11(4):384. https://doi.org/10.3390/genes11040384

Chicago/Turabian Style

Provenzano, Giovanni; Gilardoni, Angela; Maggia, Marika; Pernigo, Mattia; Sgadò, Paola; Casarosa, Simona; Bozzi, Yuri. 2020. "Altered Expression of GABAergic Markers in the Forebrain of Young and Adult Engrailed-2 Knockout Mice" Genes 11, no. 4: 384. https://doi.org/10.3390/genes11040384

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