Next Article in Journal
Transcriptomics Reveal Altered Metabolic and Signaling Pathways in Podocytes Exposed to C16 Ceramide-Enriched Lipoproteins
Previous Article in Journal
Molecular-Assisted Distinctness and Uniformity Testing Using SLAF-Sequencing Approach in Soybean
Open AccessArticle

The WUSCHELa (PtoWUSa) is Involved in Developmental Plasticity of Adventitious Root in Poplar

by Jianbo Li 1,2, Huixia Jia 2,3, Pei Sun 2,3, Jin Zhang 4, Yongxiu Xia 1, Jianjun Hu 2,3, Lijuan Wang 2,3,* and Mengzhu Lu 5,*
1
Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China
2
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
3
Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
4
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
5
College of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, Hangzhou 311300, China
*
Authors to whom correspondence should be addressed.
Genes 2020, 11(2), 176; https://doi.org/10.3390/genes11020176
Received: 8 January 2020 / Revised: 31 January 2020 / Accepted: 4 February 2020 / Published: 6 February 2020
(This article belongs to the Special Issue Tree Genetics and Improvement)
WUSCHEL-RELATED HOMEOBOX (WOX) transcription factors play critical roles in cell fate determination during plant development. As the founding member of the WOX family, WUSCHEL (WUS) is characterized for its role in maintaining stem cell in meristem. In this study, we investigated the function of Populus tomentosa WUSCHELa (PtoWUSa) in adventitious roots (ARs) in poplar. Expression profile analysis showed that PtoWUSa was not only expressed in shoot apical meristem and stem, but also expressed in ARs. Ectopic expression of PtoWUSa in Arabidopsis resulted in shortened primary root, as well as agravitropism and multiple branches. Overexpression of PtoWUSa in poplar increased the number of ARs but decreased their length. Moreover, the AR tip and lateral root tip became larger and swollen. In addition, the expression of auxin transporter genes PIN-FORMED were downregulated in ARs of transgenic plant. Taken together, these results suggest that PtoWUSa could be involved in AR development in poplar through regulating the polar auxin transport in ARs. View Full-Text
Keywords: adventitious roots; auxin; poplar; PtoWUSa; transgenic plant adventitious roots; auxin; poplar; PtoWUSa; transgenic plant
Show Figures

Figure 1

MDPI and ACS Style

Li, J.; Jia, H.; Sun, P.; Zhang, J.; Xia, Y.; Hu, J.; Wang, L.; Lu, M. The WUSCHELa (PtoWUSa) is Involved in Developmental Plasticity of Adventitious Root in Poplar. Genes 2020, 11, 176.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop