Next Article in Journal
Chromosome and Genome Divergence between the Cryptic Eurasian Malaria Vector-Species Anopheles messeae and Anopheles daciae
Next Article in Special Issue
PRL/microRNA-183/IRS1 Pathway Regulates Milk Fat Metabolism in Cow Mammary Epithelial Cells
Previous Article in Journal
Whole Genome Sequencing Indicates Heterogeneity of Hyperostotic Disorders in Dogs
Open AccessArticle

Circulatory miR-133b and miR-21 as Novel Biomarkers in Early Prediction and Diagnosis of Coronary Artery Disease

1
Dr. B R Ambedkar Center for Biomedical Research, University of Delhi, New Delhi 110007, India
2
Department of Cardiology, All India Institute of Medical Sciences, New Delhi 110029, India
3
Department of Statistics, All India Institute of Medical Sciences, New Delhi 110029, India
4
Department of Pharmacology, All India Institute of Medical Sciences, New Delhi 110029, India
*
Author to whom correspondence should be addressed.
Genes 2020, 11(2), 164; https://doi.org/10.3390/genes11020164
Received: 26 December 2019 / Revised: 30 January 2020 / Accepted: 31 January 2020 / Published: 5 February 2020
(This article belongs to the Collection microRNA Omnibus)
While coronary artery disease (CAD) has become a major threat worldwide, the timely biomarker-based early diagnosis of CAD remains a major unmet clinical challenge. We aimed towards assessing the level of circulatory microRNAs as candidates of novel biomarkers in patients with CAD. A total of 147 subjects were recruited which includes 78 subjects with angiographically proven CAD, 15 pre-atherosclerotic normal coronary artery (NCA) subjects and 54 healthy individuals. Quantitative real-time PCR assays were performed. MiR-133b was downregulated by 4.6 fold (p < 0.0001) whereas miR-21 was upregulated by ~2 fold (p < 0.0001) in plasma samples of CAD patients. Importantly, both the miRNAs showed association with disease severity as miR-133b was downregulated by 8.45 fold in acute coronary syndrome (ACS), 3.38 fold in Stable angina (SA) and 2.08 fold in NCA. MiR-21 was upregulated by 2.46 fold in ACS, 1.90 fold in SA and 1.12 fold in NCA. Moreover, miR-133b could significantly differentiate subjects with ST-elevation myocardial infarction (STEMI) from Non-STEMI. Area under the curve (AUC) for miR-133b was 0.80 with >75.6% sensitivity and specificity, AUC for miR-21 was 0.79 with >69.4% sensitivity and specificity. Our results suggest that miR-133b and miR-21 could be possible candidates of novel biomarkers in early prediction of CAD. View Full-Text
Keywords: circulatory miRNA; coronary artery disease; miR-133b; miR-21; RT-qPCR; biomarkers circulatory miRNA; coronary artery disease; miR-133b; miR-21; RT-qPCR; biomarkers
Show Figures

Figure 1

MDPI and ACS Style

Kumar, D.; Narang, R.; Sreenivas, V.; Rastogi, V.; Bhatia, J.; Saluja, D.; Srivastava, K. Circulatory miR-133b and miR-21 as Novel Biomarkers in Early Prediction and Diagnosis of Coronary Artery Disease. Genes 2020, 11, 164.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop