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Open AccessArticle

Unique and Specific m6A RNA Methylation in Mouse Embryonic and Postnatal Cerebral Cortices

1
Center for Precision Medicine, School of Medicine and School of Biomedical Sciences, Huaqiao University, Xiamen 361021, China
2
School of Life Sciences and Technology, Shanghai Jiao Tong University, Shanghai 200240, China
3
Department of Cell and Developmental Biology, Weill Medical College, Cornell University, New York, NY 10065, USA
*
Author to whom correspondence should be addressed.
Genes 2020, 11(10), 1139; https://doi.org/10.3390/genes11101139
Received: 19 August 2020 / Revised: 23 September 2020 / Accepted: 25 September 2020 / Published: 27 September 2020
(This article belongs to the Section Molecular Genetics and Genomics)
N6-methyladenosine (m6A)-mediated epitranscriptomic regulation is critical for various physiological processes. Genetic studies demonstrate that proper m6A-methylation is required for mouse brain development and function. Revealing landscapes of m6A-methylation in the cerebral cortex at different developmental stages will help to understand the biological meaning of epitranscriptomic regulation. Here, we depict the temporal-specific m6A-methylation status in mouse embryonic and postnatal cortices using methylated RNA immunoprecipitation (MeRIP) sequencing. We identified unique m6A binding motifs in stage-specific RNAs and found that more RNA transcripts are temporally methylated in embryonic cortices than in postnatal ones. Moreover, we found that cortical transcription factors and genes associated with neurological disorders are broadly as well specifically methylated at m6A sites. Our study highlights the importance of epitranscriptomic regulation in the developing cortex and provides a fundamental reference for future mechanistic examinations of m6A methylation-mediated gene expression regulation in normal brain development and neurological disorders. View Full-Text
Keywords: m6A; RNA methylation; cerebral cortex; neural development; neurological disorders m6A; RNA methylation; cerebral cortex; neural development; neurological disorders
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Zhang, L.; Du, K.; Wang, J.; Nie, Y.; Lee, T.; Sun, T. Unique and Specific m6A RNA Methylation in Mouse Embryonic and Postnatal Cerebral Cortices. Genes 2020, 11, 1139.

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