Next Article in Journal
Venous Thromboembolism in Prader–Willi Syndrome: A Questionnaire Survey
Previous Article in Journal
Computational Processing and Quality Control of Hi-C, Capture Hi-C and Capture-C Data
Open AccessArticle

Characterization of Solanum melongena Thioesterases Related to Tomato Methylketone Synthase 2

1
Faculty of Biology and Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City 700000, Vietnam
2
VN-UK Institute for Research and Executive Education, University of Danang, Danang City 550000, Vietnam
3
Agricultural Hi-tech Park, Ho Chi Minh City 700000, Vietnam
*
Author to whom correspondence should be addressed.
Genes 2019, 10(7), 549; https://doi.org/10.3390/genes10070549
Received: 15 June 2019 / Revised: 11 July 2019 / Accepted: 16 July 2019 / Published: 18 July 2019
(This article belongs to the Section Plant Genetics and Genomics)
  |  
PDF [1848 KB, uploaded 23 July 2019]
  |  

Abstract

2-Methylketones are involved in plant defense and fragrance and have industrial applications as flavor additives and for biofuel production. We isolated three genes from the crop plant Solanum melongena (eggplant) and investigated these as candidates for methylketone production. The wild tomato methylketone synthase 2 (ShMKS2), which hydrolyzes β-ketoacyl-acyl carrier proteins (ACP) to release β-ketoacids in the penultimate step of methylketone synthesis, was used as a query to identify three homologs from S. melongena: SmMKS2-1, SmMKS2-2, and SmMKS2-3. Expression and functional characterization of SmMKS2s in E. coli showed that SmMKS2-1 and SmMKS2-2 exhibited the thioesterase activity against different β-ketoacyl-ACP substrates to generate the corresponding saturated and unsaturated β-ketoacids, which can undergo decarboxylation to form their respective 2-methylketone products, whereas SmMKS2-3 showed no activity. SmMKS2-1 was expressed at high level in leaves, stems, roots, flowers, and fruits, whereas expression of SmMKS2-2 and SmMKS2-3 was mainly in flowers and fruits, respectively. Expression of SmMKS2-1 was induced in leaves by mechanical wounding, and by methyl jasmonate or methyl salicylate, but SmMKS2-2 and SmMKS2-3 genes were not induced. SmMKS2-1 is a candidate for methylketone-based defense in eggplant, and both SmMKS2-1 and SmMKS2-2 are novel MKS2 enzymes for biosynthesis of methylketones as feedstocks to biofuel production. View Full-Text
Keywords: β-ketoacids; 2-methylketones; methylketone synthase 2; methyl jasmonate; methyl salicylate; Solanum melongena; thioesterase β-ketoacids; 2-methylketones; methylketone synthase 2; methyl jasmonate; methyl salicylate; Solanum melongena; thioesterase
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Khuat, V.L.U.; Bui, V.T.T.; Tran, H.T.D.; Truong, N.X.; Nguyen, T.C.; Mai, P.H.H.; Dang, T.L.A.; Dinh, H.M.; Pham, H.T.A.; Nguyen, T.T.H. Characterization of Solanum melongena Thioesterases Related to Tomato Methylketone Synthase 2. Genes 2019, 10, 549.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top