Next Article in Journal
Adeno-Associated Viral Vectors as a Tool for Large Gene Delivery to the Retina
Previous Article in Journal
New Insights into Long Terminal Repeat Retrotransposons in Mulberry Species
Previous Article in Special Issue
Food Tracking Perspective: DNA Metabarcoding to Identify Plant Composition in Complex and Processed Food Products
Article Menu

Export Article

Open AccessArticle
Genes 2019, 10(4), 286; https://doi.org/10.3390/genes10040286

DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region

1
Biomolecular Technology Group, Leicester School of Allied Health Science, Faculty of Health and Life Sciences, De Montfort University, Leicester LE1 9BH, UK
2
National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
*
Authors to whom correspondence should be addressed.
Received: 27 February 2019 / Revised: 3 April 2019 / Accepted: 3 April 2019 / Published: 9 April 2019
(This article belongs to the Special Issue DNA Barcoding and Metabarcoding of Complex Matrices)
  |  
PDF [1352 KB, uploaded 19 April 2019]
  |  

Abstract

There is considerable potential for the use of DNA barcoding methods to authenticate raw medicinal plant materials, but their application to testing commercial products has been controversial. A simple PCR test targeting species-specific sequences within the nuclear ribosomal internal transcribed spacer (ITS) region was adapted to screen commercial products for the presence of Hypericum perforatum L. material. DNA differing widely in amount and extent of fragmentation was detected in a number of product types. Two assays were designed to further analyse this DNA using a curated database of selected Hypericum ITS sequences: A qPCR assay based on a species-specific primer pair spanning the ITS1 and ITS2 regions, using synthetic DNA reference standards for DNA quantitation and a Next Generation Sequencing (NGS) assay separately targeting the ITS1 and ITS2 regions. The ability of the assays to detect H. perforatum DNA sequences in processed medicines was investigated. Out of twenty different matrices tested, both assays detected H. perforatum DNA in five samples with more than 103 ITS copies µL−1 DNA extract, whilst the qPCR assay was also able to detect lower levels of DNA in two further samples. The NGS assay confirmed that H. perforatum was the major species in all five positive samples, though trace contaminants were also detected. View Full-Text
Keywords: Hypericum perforatum; St John’s Wort; barcoding; metabarcoding; DNA fragmentation; medicinal plant extract; qPCR Hypericum perforatum; St John’s Wort; barcoding; metabarcoding; DNA fragmentation; medicinal plant extract; qPCR
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Howard, C.; Hill, E.; Kreuzer, M.; Mali, P.; Masiero, E.; Slater, A.; Sgamma, T. DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region. Genes 2019, 10, 286.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top