Next Article in Journal
Inferring Drug-Protein–Side Effect Relationships from Biomedical Text
Next Article in Special Issue
Physiological and Genetic Dissection of Sucrose Inputs to the Arabidopsis thaliana Circadian System
Previous Article in Journal
Genome-Wide Identification and Analysis of High-Copy-Number LTR Retrotransposons in Asian Pears
Previous Article in Special Issue
Transcriptome Analysis of Diurnal Gene Expression in Chinese Cabbage
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle

A Magnesium Transport Protein Related to Mammalian SLC41 and Bacterial MgtE Contributes to Circadian Timekeeping in a Unicellular Green Alga

School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK
*
Author to whom correspondence should be addressed.
Genes 2019, 10(2), 158; https://doi.org/10.3390/genes10020158
Received: 7 January 2019 / Revised: 29 January 2019 / Accepted: 12 February 2019 / Published: 19 February 2019
(This article belongs to the Special Issue Genetic Regulation of Circadian Rhythm in Plants)
  |  
PDF [4354 KB, uploaded 23 February 2019]
  |     |  

Abstract

Circadian clocks in eukaryotes involve both transcriptional-translational feedback loops, post-translational regulation, and metabolic, non-transcriptional oscillations. We recently identified the involvement of circadian oscillations in the intracellular concentrations of magnesium ions ([Mg2+]i) that were conserved in three eukaryotic kingdoms. [Mg2+]i in turn contributes to transcriptional clock properties of period and amplitude, and can function as a zeitgeber to define phase. However, the mechanism—or mechanisms—responsible for the generation of [Mg2+]i oscillations, and whether these are functionally conserved across taxonomic groups, remain elusive. We employed the cellular clock model Ostreococcus tauri to provide a first study of an MgtE domain-containing protein in the green lineage. OtMgtE shares homology with the mammalian SLC41A1 magnesium/sodium antiporter, which has previously been implicated in maintaining clock period. Using genetic overexpression, we found that OtMgtE contributes to both timekeeping and daily changes in [Mg2+]i. However, pharmacological experiments and protein sequence analyses indicated that critical differences exist between OtMgtE and either the ancestral MgtE channel or the mammalian SLC41 antiporters. We concluded that even though MgtE domain-containing proteins are only distantly related, these proteins retain a shared role in contributing to cellular timekeeping and the regulation of [Mg2+]i. View Full-Text
Keywords: magnesium transport; circadian clocks; cellular rhythms; transporter proteins; Ostreococcus tauri magnesium transport; circadian clocks; cellular rhythms; transporter proteins; Ostreococcus tauri
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Feord, H.K.; Dear, F.E.; Obbard, D.J.; van Ooijen, G. A Magnesium Transport Protein Related to Mammalian SLC41 and Bacterial MgtE Contributes to Circadian Timekeeping in a Unicellular Green Alga. Genes 2019, 10, 158.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top