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10.3390/genes10020103
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Article

De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species

by
Xing Huang
1,
Mei Xiao
2,
Jingen Xi
1,
Chunping He
1,
Jinlong Zheng
1,
Helong Chen
3,
Jianming Gao
3,
Shiqing Zhang
3,
Weihuai Wu
1,
Yanqiong Liang
1,
Li Xie
4 and
Kexian Yi
1,*
1
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
2
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
3
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
4
Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(2), 103; https://doi.org/10.3390/genes10020103
Submission received: 28 November 2018 / Revised: 19 January 2019 / Accepted: 28 January 2019 / Published: 30 January 2019
(This article belongs to the Special Issue Genetics, genomics, and evolution of CAM photosynthesis)
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Abstract

Agave plants are important crassulacean acid metabolism (CAM) plants with multiple agricultural uses, such as being used in tequila and fiber production. Agave hybrid H11648 ((A. amaniensis Trel. and Nowell × A. angustifolia Haw.) × A. amaniensis) is the main cultivated Agave species for fiber production in large tropical areas around the world. In this study, we conducted a transcriptome analysis of A. H11648. About 49.25 million clean reads were obtained by Illumina paired-end sequencing. De novo assembly produced 148,046 unigenes with more than 40% annotated in public databases, or matched homologs in model plants. More homologous gene pairs were found in Asparagus genome than in Arabidopsis or rice, which indicated a close evolutionary relationship between Asparagus and A. H11648. CAM-related gene families were also characterized as previously reported in A. americana. We further identified 12 cellulose synthase genes (CesA) in Asparagus genome and 38 CesA sequences from A. H11648, A. americana, A. deserti and A. tequilana. The full-length CesA genes were used as references for the cloning and assembly of their homologs in other Agave species. As a result, we obtained CesA1/3/4/5/7 genes with full-length coding region in the four Agave species. Phylogenetic and expression analysis revealed a conserved evolutionary pattern, which could not explain the distinct fiber traits in different Agave species. We inferred that transcriptional regulation might be responsible for Agave fiber development. This study represents the transcriptome of A. H11648, which would expand the number of Agave genes and benefit relevant studies of Agave fiber development.
Keywords: RNA-seq; crassulacean acid metabolism; Agave; cellulose synthase; Asparagus; gene clone; phylogeny; gene expression RNA-seq; crassulacean acid metabolism; Agave; cellulose synthase; Asparagus; gene clone; phylogeny; gene expression

Share and Cite

MDPI and ACS Style

Huang, X.; Xiao, M.; Xi, J.; He, C.; Zheng, J.; Chen, H.; Gao, J.; Zhang, S.; Wu, W.; Liang, Y.; et al. De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species. Genes 2019, 10, 103. https://doi.org/10.3390/genes10020103

AMA Style

Huang X, Xiao M, Xi J, He C, Zheng J, Chen H, Gao J, Zhang S, Wu W, Liang Y, et al. De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species. Genes. 2019; 10(2):103. https://doi.org/10.3390/genes10020103

Chicago/Turabian Style

Huang, Xing, Mei Xiao, Jingen Xi, Chunping He, Jinlong Zheng, Helong Chen, Jianming Gao, Shiqing Zhang, Weihuai Wu, Yanqiong Liang, and et al. 2019. "De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species" Genes 10, no. 2: 103. https://doi.org/10.3390/genes10020103

APA Style

Huang, X., Xiao, M., Xi, J., He, C., Zheng, J., Chen, H., Gao, J., Zhang, S., Wu, W., Liang, Y., Xie, L., & Yi, K. (2019). De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species. Genes, 10(2), 103. https://doi.org/10.3390/genes10020103

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