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Article

Distinct Roles for RAB10 and RAB29 in Pathogenic LRRK2-Mediated Endolysosomal Trafficking Alterations

1
Institute of Parasitology and Biomedicine “López-Neyra”, Consejo Superior de Investigaciones Científicas (CSIC), Avda del Conocimiento s/n, 18016 Granada, Spain
2
Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
3
Department of Anesthesiology, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ 07103, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Cells 2020, 9(7), 1719; https://doi.org/10.3390/cells9071719
Received: 23 June 2020 / Revised: 12 July 2020 / Accepted: 13 July 2020 / Published: 17 July 2020
(This article belongs to the Special Issue LRRK2-Dependent Neurodegeneration in Parkinson’s Disease)
Mutations in the gene encoding leucine-rich repeat kinase 2 (LRRK2) cause familial Parkinson’s disease, and sequence variations are associated with the sporadic form of the disease. LRRK2 phosphorylates a subset of RAB proteins implicated in secretory and recycling trafficking pathways, including RAB8A and RAB10. Another RAB protein, RAB29, has been reported to recruit LRRK2 to the Golgi, where it stimulates its kinase activity. Our previous studies revealed that G2019S LRRK2 expression or knockdown of RAB8A deregulate epidermal growth factor receptor (EGFR) trafficking, with a concomitant accumulation of the receptor in a RAB4-positive recycling compartment. Here, we show that the G2019S LRRK2-mediated EGFR deficits are mimicked by knockdown of RAB10 and rescued by expression of active RAB10. By contrast, RAB29 knockdown is without effect, but expression of RAB29 also rescues the pathogenic LRRK2-mediated trafficking deficits independently of Golgi integrity. Our data suggest that G2019S LRRK2 deregulates endolysosomal trafficking by impairing the function of RAB8A and RAB10, while RAB29 positively modulates non-Golgi-related trafficking events impaired by pathogenic LRRK2. View Full-Text
Keywords: Parkinson’s disease; LRRK2; RAB10; RAB29; endolysosome; Golgi Parkinson’s disease; LRRK2; RAB10; RAB29; endolysosome; Golgi
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MDPI and ACS Style

Rivero-Ríos, P.; Romo-Lozano, M.; Fernández, B.; Fdez, E.; Hilfiker, S. Distinct Roles for RAB10 and RAB29 in Pathogenic LRRK2-Mediated Endolysosomal Trafficking Alterations. Cells 2020, 9, 1719. https://doi.org/10.3390/cells9071719

AMA Style

Rivero-Ríos P, Romo-Lozano M, Fernández B, Fdez E, Hilfiker S. Distinct Roles for RAB10 and RAB29 in Pathogenic LRRK2-Mediated Endolysosomal Trafficking Alterations. Cells. 2020; 9(7):1719. https://doi.org/10.3390/cells9071719

Chicago/Turabian Style

Rivero-Ríos, Pilar, Maria Romo-Lozano, Belén Fernández, Elena Fdez, and Sabine Hilfiker. 2020. "Distinct Roles for RAB10 and RAB29 in Pathogenic LRRK2-Mediated Endolysosomal Trafficking Alterations" Cells 9, no. 7: 1719. https://doi.org/10.3390/cells9071719

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