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Open AccessArticle

A Clonal NG2-Glia Cell Response in a Mouse Model of Multiple Sclerosis

1
Departamento de Neurobiología Molecular, Celular y del Desarrollo, Instituto Cajal-CSIC, 28002 Madrid, Spain
2
Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Departamento de Neurociencias, Universidad del País Vasco, 48940 Leioa, Spain
3
Achucarro Basque Center for Neuroscience, 48940 Leioa, Spain
*
Author to whom correspondence should be addressed.
Cells 2020, 9(5), 1279; https://doi.org/10.3390/cells9051279
Received: 8 April 2020 / Revised: 17 May 2020 / Accepted: 19 May 2020 / Published: 21 May 2020
NG2-glia, also known as oligodendrocyte precursor cells (OPCs), have the potential to generate new mature oligodendrocytes and thus, to contribute to tissue repair in demyelinating diseases like multiple sclerosis (MS). Once activated in response to brain damage, NG2-glial cells proliferate, and they acquire a reactive phenotype and a heterogeneous appearance. Here, we set out to investigate the distribution and phenotypic diversity of NG2-glia relative to their ontogenic origin, and whether there is a clonal NG2-glial response to lesion in an experimental autoimmune encephalomyelitis (EAE) murine model of MS. As such, we performed in utero electroporation of the genomic lineage tracer, StarTrack, to follow the fate of NG2-glia derived from single progenitors and to evaluate their response to brain damage after EAE induction. We then analyzed the dispersion of the NG2-glia derived clonally from single pallial progenitors in the brain of EAE mice. In addition, we examined several morphological parameters to assess the degree of NG2-glia reactivity in clonally-related cells. Our results reveal the heterogeneity of these progenitors and their cell progeny in a scenario of autoimmune demyelination, revealing the ontogenic phenomena at play in these processes. View Full-Text
Keywords: NG2-glia; progenitors; multiple sclerosis; lineage; in utero electroporation; morphometric analyses; clonal analyses; lesioned brain NG2-glia; progenitors; multiple sclerosis; lineage; in utero electroporation; morphometric analyses; clonal analyses; lesioned brain
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MDPI and ACS Style

Barriola, S.; Pérez-Cerdá, F.; Matute, C.; Bribián, A.; López-Mascaraque, L. A Clonal NG2-Glia Cell Response in a Mouse Model of Multiple Sclerosis. Cells 2020, 9, 1279.

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