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Review

A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping

1
Institute of Biostructures and Bioimaging, IBB, CNR, 80134 Naples, Italy
2
Institute of Molecular Genetics, IGM, CNR, 27100 Pavia, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2020, 9(5), 1267; https://doi.org/10.3390/cells9051267
Received: 24 April 2020 / Revised: 14 May 2020 / Accepted: 19 May 2020 / Published: 20 May 2020
The current coronavirus disease-2019 (COVID-19) pandemic is due to the novel coronavirus SARS-CoV-2. The scientific community has mounted a strong response by accelerating research and innovation, and has quickly set the foundation for understanding the molecular determinants of the disease for the development of targeted therapeutic interventions. The replication of the viral genome within the infected cells is a key stage of the SARS-CoV-2 life cycle. It is a complex process involving the action of several viral and host proteins in order to perform RNA polymerization, proofreading and final capping. This review provides an update of the structural and functional data on the key actors of the replicatory machinery of SARS-CoV-2, to fill the gaps in the currently available structural data, which is mainly obtained through homology modeling. Moreover, learning from similar viruses, we collect data from the literature to reconstruct the pattern of interactions among the protein actors of the SARS-CoV-2 RNA polymerase machinery. Here, an important role is played by co-factors such as Nsp8 and Nsp10, not only as allosteric activators but also as molecular connectors that hold the entire machinery together to enhance the efficiency of RNA replication. View Full-Text
Keywords: SARS-CoV-2; COVID19; RNA replication; protein structure; infectious disease SARS-CoV-2; COVID19; RNA replication; protein structure; infectious disease
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MDPI and ACS Style

Romano, M.; Ruggiero, A.; Squeglia, F.; Maga, G.; Berisio, R. A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping. Cells 2020, 9, 1267. https://doi.org/10.3390/cells9051267

AMA Style

Romano M, Ruggiero A, Squeglia F, Maga G, Berisio R. A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping. Cells. 2020; 9(5):1267. https://doi.org/10.3390/cells9051267

Chicago/Turabian Style

Romano, Maria, Alessia Ruggiero, Flavia Squeglia, Giovanni Maga, and Rita Berisio. 2020. "A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping" Cells 9, no. 5: 1267. https://doi.org/10.3390/cells9051267

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