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Article

Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands

1
Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA
2
Department of Otolaryngology-Head and Neck Surgery, School of Medicine, University of Missouri, Columbia, MO 65212, USA
3
Cell Sheet Tissue Engineering Center (CSTEC), Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112, USA
4
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo 162-8666, Japan
5
Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA
*
Author to whom correspondence should be addressed.
Cells 2020, 9(12), 2645; https://doi.org/10.3390/cells9122645
Received: 16 November 2020 / Revised: 5 December 2020 / Accepted: 7 December 2020 / Published: 9 December 2020
Thermoresponsive cell culture plates release cells as confluent living sheets in response to small changes in temperature, with recovered cell sheets retaining functional extracellular matrix proteins and tight junctions, both of which indicate formation of intact and functional tissue. Our recent studies demonstrated that cell sheets are highly effective in promoting mouse submandibular gland (SMG) cell differentiation and recovering tissue integrity. However, these studies were performed only at early time points and extension of the observation period is needed to investigate duration of the cell sheets. Thus, the goal of this study was to demonstrate that treatment of wounded mouse SMG with cell sheets is capable of increasing salivary epithelial integrity over extended time periods. The results indicate that cell sheets promote tissue organization as early as eight days after transplantation and that these effects endure through Day 20. Furthermore, cell sheet transplantation in wounded SMG induces a significant time-dependent enhancement of cell polarization, differentiation and ion transporter expression. Finally, this treatment restored saliva quantity to pre-wounding levels at both eight and twenty days post-surgery and significantly improved saliva quality at twenty days post-surgery. These data indicate that cell sheets engineered with thermoresponsive cell culture plates are useful for salivary gland regeneration and provide evidence for the long-term stability of cell sheets, thereby offering a potential new therapeutic strategy for treating hyposalivation. View Full-Text
Keywords: cell sheet; extracellular matrix; hyposalivation; regeneration; wound healing cell sheet; extracellular matrix; hyposalivation; regeneration; wound healing
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MDPI and ACS Style

dos Santos, H.T.; Kim, K.; Okano, T.; Camden, J.M.; Weisman, G.A.; Baker, O.J.; Nam, K. Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands. Cells 2020, 9, 2645. https://doi.org/10.3390/cells9122645

AMA Style

dos Santos HT, Kim K, Okano T, Camden JM, Weisman GA, Baker OJ, Nam K. Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands. Cells. 2020; 9(12):2645. https://doi.org/10.3390/cells9122645

Chicago/Turabian Style

dos Santos, Harim T., Kyungsook Kim, Teruo Okano, Jean M. Camden, Gary A. Weisman, Olga J. Baker, and Kihoon Nam. 2020. "Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands" Cells 9, no. 12: 2645. https://doi.org/10.3390/cells9122645

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