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Open AccessArticle

Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors

1
Laboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology-CIBIO, University of Trento, 38123 Trento, Italy
2
Fondazione Bruno Kessler-Center for Material and Microsystem, 38123 Trento, Italy
3
Laboratory of Neural Development and Regeneration, Department of Cellular, Computational and Integrative Biology-CIBIO, University of Trento, 38123 Trento, Italy
4
Advanced Imaging Facility, Department of Cellular, Computational and Integrative Biology-CIBIO, University of Trento, 38123 Trento, Italy
5
Laboratory of Bio-Inspired and Graphene Nanomechanics, Department of Civil, Environmental and Mechanical Engineering, University of Trento, 38123 Trento, Italy
6
School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, UK
7
Ket-Lab, Edoardo Amaldi Foundation, via del Politecnico snc, I-00133 Roma, Italy
*
Authors to whom correspondence should be addressed.
A.C. and S.G. contribute equally to this work and share first authorship.
M.B. and L.C. contribute equally to this work and share last authorship.
Cells 2020, 9(1), 88; https://doi.org/10.3390/cells9010088
Received: 25 November 2019 / Revised: 18 December 2019 / Accepted: 23 December 2019 / Published: 30 December 2019
(This article belongs to the Special Issue 3D Stem Cell Culture)
Silicon is a promising material for tissue engineering since it allows to produce micropatterned scaffolding structures resembling biological tissues. Using specific fabrication methods, it is possible to build aligned 3D network-like structures. In the present study, we exploited vertically-aligned silicon micropillar arrays as culture systems for human iPSC-derived cortical progenitors. In particular, our aim was to mimic the radially-oriented cortical radial glia fibres that during embryonic development play key roles in controlling the expansion, radial migration and differentiation of cortical progenitors, which are, in turn, pivotal to the establishment of the correct multilayered cerebral cortex structure. Here we show that silicon vertical micropillar arrays efficiently promote expansion and stemness preservation of human cortical progenitors when compared to standard monolayer growth conditions. Furthermore, the vertically-oriented micropillars allow the radial migration distinctive of cortical progenitors in vivo. These results indicate that vertical silicon micropillar arrays can offer an optimal system for human cortical progenitors’ growth and migration. Furthermore, similar structures present an attractive platform for cortical tissue engineering. View Full-Text
Keywords: human cortical progenitors; 3D culture; silicon pillars; cell growth; hiPSC-derived neural progenitors; cerebral cortex human cortical progenitors; 3D culture; silicon pillars; cell growth; hiPSC-derived neural progenitors; cerebral cortex
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MDPI and ACS Style

Cutarelli, A.; Ghio, S.; Zasso, J.; Speccher, A.; Scarduelli, G.; Roccuzzo, M.; Crivellari, M.; Maria Pugno, N.; Casarosa, S.; Boscardin, M.; Conti, L. Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors. Cells 2020, 9, 88. https://doi.org/10.3390/cells9010088

AMA Style

Cutarelli A, Ghio S, Zasso J, Speccher A, Scarduelli G, Roccuzzo M, Crivellari M, Maria Pugno N, Casarosa S, Boscardin M, Conti L. Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors. Cells. 2020; 9(1):88. https://doi.org/10.3390/cells9010088

Chicago/Turabian Style

Cutarelli, Alessandro; Ghio, Simone; Zasso, Jacopo; Speccher, Alessandra; Scarduelli, Giorgina; Roccuzzo, Michela; Crivellari, Michele; Maria Pugno, Nicola; Casarosa, Simona; Boscardin, Maurizio; Conti, Luciano. 2020. "Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors" Cells 9, no. 1: 88. https://doi.org/10.3390/cells9010088

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