Plasma Fibrinogen-Like 1 as a Potential Biomarker for Radiation-Induced Liver Injury
Abstract
:1. Introduction
2. Experimental Section
2.1. Animal Experiments
2.2. Liver Imaging
2.3. Irradiation
2.4. Sample Preparation
2.5. Histopathological Analysis
2.6. Western Blotting
2.7. Cell Culture and Reagents
2.8. Quantitative Real-Time Reverse Transcription-PCR (RT-qPCR)
2.9. ELISA
2.10. Statistical Analysis
3. Results
3.1. Up-Regulation of FGL1 in Mouse Liver by Irradiation
3.1.1. Association between FGL1 and Radiation-Induced Liver Injury
3.1.2. Up-Regulation of FGL1 and 8-OHdG in the Liver at the Subacute Phase after IR
3.2. Up-Regulation of FGL1 in Human Hepatocytes Responding to Radiation
3.2.1. FGL1 Expression in HHs Following IR
3.2.2. FGL1 Upregulation by Radiation or Oxidative Stress from Hydrogen Peroxidase in HHs
3.3. Plasma FGL1 as a Biomarker for Radiation Liver Injury
3.3.1. FGL1 Expression in the Liver and Plasma Following Total Body Exposure
3.3.2. FGL1 Expression in the Liver and Plasma Following Liver IR
3.3.3. Association between Plasma FGL1 and the Radiation Dose or Irradiation Field Size Relative to the Liver
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Han, N.-K.; Jung, M.G.; Jeong, Y.J.; Son, Y.; Han, S.C.; Park, S.; Lim, Y.-B.; Lee, Y.-J.; Kim, S.-H.; Park, S.C.; et al. Plasma Fibrinogen-Like 1 as a Potential Biomarker for Radiation-Induced Liver Injury. Cells 2019, 8, 1042. https://doi.org/10.3390/cells8091042
Han N-K, Jung MG, Jeong YJ, Son Y, Han SC, Park S, Lim Y-B, Lee Y-J, Kim S-H, Park SC, et al. Plasma Fibrinogen-Like 1 as a Potential Biomarker for Radiation-Induced Liver Injury. Cells. 2019; 8(9):1042. https://doi.org/10.3390/cells8091042
Chicago/Turabian StyleHan, Na-Kyung, Myung Gu Jung, Ye Ji Jeong, Yeonghoon Son, Su Chul Han, Seungwoo Park, Young-Bin Lim, Yoon-Jin Lee, Sung-Ho Kim, Su Cheol Park, and et al. 2019. "Plasma Fibrinogen-Like 1 as a Potential Biomarker for Radiation-Induced Liver Injury" Cells 8, no. 9: 1042. https://doi.org/10.3390/cells8091042