Supramolecular Structures of the Dictyostelium Lamin NE81
1
Department of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, Germany
2
Department of Animal Physiology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, Germany
3
Department of Biosciences, Durham University, Science Laboratories, South Road, Durham DH1 3LE, UK
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(2), 162; https://doi.org/10.3390/cells8020162
Received: 31 January 2019
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Revised: 12 February 2019
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Accepted: 15 February 2019
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Published: 16 February 2019
(This article belongs to the Special Issue Simple Organisms for Complex Problems: Modeling Human Disease in Yeast and Dictyostelium)
Nuclear lamins are nucleus-specific intermediate filaments (IF) found at the inner nuclear membrane (INM) of the nuclear envelope (NE). Together with nuclear envelope transmembrane proteins, they form the nuclear lamina and are crucial for gene regulation and mechanical robustness of the nucleus and the whole cell. Recently, we characterized Dictyostelium NE81 as an evolutionarily conserved lamin-like protein, both on the sequence and functional level. Here, we show on the structural level that the Dictyostelium NE81 is also capable of assembling into filaments, just as metazoan lamin filament assemblies. Using field-emission scanning electron microscopy, we show that NE81 expressed in Xenopous oocytes forms filamentous structures with an overall appearance highly reminiscent of Xenopus lamin B2. The in vitro assembly properties of recombinant His-tagged NE81 purified from Dictyostelium extracts are very similar to those of metazoan lamins. Super-resolution stimulated emission depletion (STED) and expansion microscopy (ExM), as well as transmission electron microscopy of negatively stained purified NE81, demonstrated its capability of forming filamentous structures under low-ionic-strength conditions. These results recommend Dictyostelium as a non-mammalian model organism with a well-characterized nuclear envelope involving all relevant protein components known in animal cells.
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Keywords:
lamin; NE81; Dictyostelium; nuclear envelope; nuclear lamina; expansion microscopy
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MDPI and ACS Style
Grafe, M.; Batsios, P.; Meyer, I.; Lisin, D.; Baumann, O.; Goldberg, M.W.; Gräf, R. Supramolecular Structures of the Dictyostelium Lamin NE81. Cells 2019, 8, 162. https://doi.org/10.3390/cells8020162
AMA Style
Grafe M, Batsios P, Meyer I, Lisin D, Baumann O, Goldberg MW, Gräf R. Supramolecular Structures of the Dictyostelium Lamin NE81. Cells. 2019; 8(2):162. https://doi.org/10.3390/cells8020162
Chicago/Turabian StyleGrafe, Marianne, Petros Batsios, Irene Meyer, Daria Lisin, Otto Baumann, Martin W. Goldberg, and Ralph Gräf. 2019. "Supramolecular Structures of the Dictyostelium Lamin NE81" Cells 8, no. 2: 162. https://doi.org/10.3390/cells8020162
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