Next Article in Journal
The Emerging Role of Gβ Subunits in Human Genetic Diseases
Next Article in Special Issue
Epithelial to Mesenchymal Transition: A Mechanism that Fuels Cancer Radio/Chemoresistance
Previous Article in Journal
Transthyretin Maintains Muscle Homeostasis through the Novel Shuttle Pathway of Thyroid Hormones during Myoblast Differentiation
Previous Article in Special Issue
Development of a Stromal Microenvironment Experimental Model Containing Proto-Myofibroblast Like Cells and Analysis of Its Crosstalk with Melanoma Cells: A New Tool to Potentiate and Stabilize Tumor Suppressor Phenotype of Dermal Myofibroblasts
Article Menu
Open AccessArticle

Stromal Cell Signature Associated with Response to Neoadjuvant Chemotherapy in Locally Advanced Breast Cancer

Departamento de Radiologia e Oncologia, Centro de Investigação Translacional em Oncologia, Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-000, SP, Brazil
Departamento de Mastologia, Hospital de Câncer de Barretos, Barretos 14.784-400, SP, Brazil
A.C. Camargo Cancer Center, Sao Paulo 01525-001, SP, Brazil
Departamento de Patologia, Hospital de Câncer de Barretos, Barretos 14.784-400, SP, Brazil
Departamento de Estatística, Centro de Ciências Exatas e de Tecnologia, Universidade Federal de São Carlos, Sao Carlos 13565-905, SP, Brazil
Department of Mathematics and Statistics, The University of Western Australia, M019, 35 Stirling Highway, 6009 Crawley, WA, Australia
Departamento de Estatística, Instituto de Matemática e Estatística, Universidade de São Paulo, São Paulo 05508-090, SP, Brazil
Author to whom correspondence should be addressed.
These authors participated equally in this work.
Cells 2019, 8(12), 1566;
Received: 23 September 2019 / Revised: 27 October 2019 / Accepted: 31 October 2019 / Published: 4 December 2019
(This article belongs to the Special Issue Plasticity in Cancer and in Microenvironmental Cells)
Breast cancer stromal compartment, may influence responsiveness to chemotherapy. Our aim was to detect a stromal cell signature (using a direct approach of microdissected stromal cells) associated with response to neoadjuvant chemotherapy (neoCT) in locally advanced breast cancer (LABC). The tumor samples were collected from 44 patients with LABC (29 estrogen receptor (ER) positive and 15 ER negative) before the start of any treatment. Neoadjuvant chemotherapy consisted of doxorubicin and cyclophosphamide, followed by paclitaxel. Response was defined as downstaging to maximum ypT1a-b/ypN0. The stromal cells, mainly composed of fibroblast and immune cells, were microdissected from fresh frozen tumor samples and gene expression profile was determined using Agilent SurePrint G3 Human Gene Expression microarrays. Expression levels were compared using MeV (MultiExperiment Viewer) software, applying SAM (significance analysis of microarrays). To classify samples according to tumor response, the order of median based on confidence statements (MedOr) was used, and to identify gene sets correlated with the phenotype downstaging, gene set enrichment analysis (GSEA). Nine patients presented disease downstaging. Eleven sequences (FDR 17) were differentially expressed, all of which (except H2AFJ) more expressed in responsive tumors, including PTCHD1 and genes involved in abnormal cytotoxic T cell physiology, TOX, LY75, and SH2D1A. The following four pairs of markers could correctly classify all tumor samples according to response: PTCHD1/PDXDC2P, LOC100506731/NEURL4, SH2D1A/ENST00000478672, and TOX/H2AFJ. Gene sets correlated with tumor downstaging (FDR < 0.01) were mainly involved in immune response or lymphocyte activation, including CD47, LCK, NCK1, CD24, CD3E, ZAP70, FOXP3, and CD74, among others. In locally advanced breast cancer, stromal cells may present specific features of immune response that may be associated with chemotherapy response. View Full-Text
Keywords: breast cancer; stromal cells; gene expression; chemotherapy neoadjuvant breast cancer; stromal cells; gene expression; chemotherapy neoadjuvant
Show Figures

Figure 1

MDPI and ACS Style

Katayama, M.L.H.; Vieira, R.A.C.; Andrade, V.P.; Roela, R.A.; Lima, L.G.C.A.; Kerr, L.M.; Campos, A.P.; Pereira, C.A.B.; Serio, P.A.M.P.; Encinas, G.; Maistro, S.; Petroni, M.A.L.; Brentani, M.M.; Folgueira, M.A.A.K. Stromal Cell Signature Associated with Response to Neoadjuvant Chemotherapy in Locally Advanced Breast Cancer. Cells 2019, 8, 1566.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop