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Article

Commonly Used Pancreatic Stellate Cell Cultures Differ Phenotypically and in Their Interactions with Pancreatic Cancer Cells

1
Department of Pathology, Institute of Clinical Medicine, University of Oslo, Blindern, 0316 Oslo, Norway
2
Department of Pharmacology, Institute of Clinical Medicine, University of Oslo, Blindern, 0316 Oslo, Norway
3
Department of Pathology and Molecular Pathology, University Hospital Zürich, University of Zürich, Schmelzbergstrasse 12, 8091 Zürich, Switzerland
4
Department of Hepato-Pancreato-Biliary Surgery, Institute of Clinical Medicine, University of Oslo, P.O. Box 1171 Blindern, 0318 Oslo, Norway
5
Department of Pathology, Oslo University Hospital Rikshospitalet, Nydalen, 0424 Oslo, Norway
6
Department of Clinical Science, Intervention and Technology, Karolinska Institutet, K 53, 141 86 Stockholm, Sweden
7
Department of Hepato-Pancreato-Biliary Surgery, Oslo University Hospital Rikshospitalet, Nydalen, 0424 Oslo, Norway
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2019, 8(1), 23; https://doi.org/10.3390/cells8010023
Received: 26 November 2018 / Revised: 24 December 2018 / Accepted: 28 December 2018 / Published: 5 January 2019
Activated pancreatic stellate cells (PSCs) play a central role in the tumor stroma of pancreatic ductal adenocarcinoma (PDAC). Given the limited availability of patient-derived PSCs from PDAC, immortalized PSC cell lines of murine and human origin have been established; however, it is not elucidated whether differences in species, organ disease status, donor age, and immortalization alter the PSC phenotype and behavior compared to that of patient-derived primary PSC cultures. Therefore, a panel of commonly used PSC cultures was examined for important phenotypical and functional features: three primary cultures from human PDAC, one primary from normal human pancreas, and three immortalized (one from human, two from murine pancreas). Growth rate was considerably lower in primary PSCs from human PDAC. Basal collagen synthesis varied between the PSC cultures, and TGF-β stimulation increased collagen synthesis only in non-immortalized cultures. Differences in secretome composition were observed along with a divergence in the DNA synthesis, migration, and response to gemcitabine of PDAC cell lines that were grown in conditioned medium from the various PSC cultures. The findings reveal considerable differences in features and functions that are key to PSCs and in the interactions with PDAC. These observations may be relevant to researchers when selecting the most appropriate PSC culture for their experiments. View Full-Text
Keywords: pancreatic stellate cells; pancreatic cancer; tumor-stroma interaction; TGF-β pancreatic stellate cells; pancreatic cancer; tumor-stroma interaction; TGF-β
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MDPI and ACS Style

Lenggenhager, D.; Amrutkar, M.; Sántha, P.; Aasrum, M.; Löhr, J.-M.; Gladhaug, I.P.; Verbeke, C.S. Commonly Used Pancreatic Stellate Cell Cultures Differ Phenotypically and in Their Interactions with Pancreatic Cancer Cells. Cells 2019, 8, 23. https://doi.org/10.3390/cells8010023

AMA Style

Lenggenhager D, Amrutkar M, Sántha P, Aasrum M, Löhr J-M, Gladhaug IP, Verbeke CS. Commonly Used Pancreatic Stellate Cell Cultures Differ Phenotypically and in Their Interactions with Pancreatic Cancer Cells. Cells. 2019; 8(1):23. https://doi.org/10.3390/cells8010023

Chicago/Turabian Style

Lenggenhager, Daniela, Manoj Amrutkar, Petra Sántha, Monica Aasrum, Johannes-Matthias Löhr, Ivar P. Gladhaug, and Caroline S. Verbeke. 2019. "Commonly Used Pancreatic Stellate Cell Cultures Differ Phenotypically and in Their Interactions with Pancreatic Cancer Cells" Cells 8, no. 1: 23. https://doi.org/10.3390/cells8010023

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