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Open AccessArticle

B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody

1
Research & Development Department, Cellular Technology Limited, Shaker Heights, OH 44122, USA
2
Institute of Anatomy and Cell Biology, Friedrich-Alexander University Erlangen-Nürnberg, 91054 Erlangen, Germany
*
Author to whom correspondence should be addressed.
Cells 2018, 7(6), 50; https://doi.org/10.3390/cells7060050
Received: 6 April 2018 / Revised: 2 May 2018 / Accepted: 22 May 2018 / Published: 31 May 2018
(This article belongs to the Special Issue Recent Advances in ELISPOT Research)
In individuals who have once developed humoral immunity to an infectious/foreign antigen, the antibodies present in their body can mediate instant protection when the antigen re-enters. Such antigen-specific antibodies can be readily detected in the serum. Long term humoral immunity is, however, also critically dependent on the ability of memory B cells to engage in a secondary antibody response upon re-exposure to the antigen. Antibody molecules in the body are short lived, having a half-life of weeks, while memory B cells have a life span of decades. Therefore, the presence of serum antibodies is not always a reliable indicator of B cell memory and comprehensive monitoring of humoral immunity requires that both serum antibodies and memory B cells be assessed. The prevailing view is that resting memory B cells and B cell blasts in peripheral blood mononuclear cells (PBMC) cannot be cryopreserved without losing their antibody secreting function, and regulated high throughput immune monitoring of B cell immunity is therefore confined to—and largely limited by—the need to test freshly isolated PBMC. Using optimized protocols for freezing and thawing of PBMC, and four color ImmunoSpot® analysis for the simultaneous detection of all immunoglobulin classes/subclasses we show here that both resting memory B cells and B cell blasts retain their ability to secrete antibody after thawing, and thus demonstrate the feasibility of B cell immune monitoring using cryopreserved PBMC. View Full-Text
Keywords: four color B cell ELISPOT; immune monitoring; freeze-thawing PBMC; plasma cells; antibody secretion; immunoglobulins; antibodies; immunoglobulin classes and subclasses; antibody-secreting cells; IgA; IgE; IgD; IgM; IgG1; IgG2; IgG3; IgG4; multiplex immune assay four color B cell ELISPOT; immune monitoring; freeze-thawing PBMC; plasma cells; antibody secretion; immunoglobulins; antibodies; immunoglobulin classes and subclasses; antibody-secreting cells; IgA; IgE; IgD; IgM; IgG1; IgG2; IgG3; IgG4; multiplex immune assay
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MDPI and ACS Style

Fecher, P.; Caspell, R.; Naeem, V.; Karulin, A.Y.; Kuerten, S.; Lehmann, P.V. B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody. Cells 2018, 7, 50. https://doi.org/10.3390/cells7060050

AMA Style

Fecher P, Caspell R, Naeem V, Karulin AY, Kuerten S, Lehmann PV. B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody. Cells. 2018; 7(6):50. https://doi.org/10.3390/cells7060050

Chicago/Turabian Style

Fecher, Philipp; Caspell, Richard; Naeem, Villian; Karulin, Alexey Y.; Kuerten, Stefanie; Lehmann, Paul V. 2018. "B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody" Cells 7, no. 6: 50. https://doi.org/10.3390/cells7060050

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