B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody
AbstractIn individuals who have once developed humoral immunity to an infectious/foreign antigen, the antibodies present in their body can mediate instant protection when the antigen re-enters. Such antigen-specific antibodies can be readily detected in the serum. Long term humoral immunity is, however, also critically dependent on the ability of memory B cells to engage in a secondary antibody response upon re-exposure to the antigen. Antibody molecules in the body are short lived, having a half-life of weeks, while memory B cells have a life span of decades. Therefore, the presence of serum antibodies is not always a reliable indicator of B cell memory and comprehensive monitoring of humoral immunity requires that both serum antibodies and memory B cells be assessed. The prevailing view is that resting memory B cells and B cell blasts in peripheral blood mononuclear cells (PBMC) cannot be cryopreserved without losing their antibody secreting function, and regulated high throughput immune monitoring of B cell immunity is therefore confined to—and largely limited by—the need to test freshly isolated PBMC. Using optimized protocols for freezing and thawing of PBMC, and four color ImmunoSpot® analysis for the simultaneous detection of all immunoglobulin classes/subclasses we show here that both resting memory B cells and B cell blasts retain their ability to secrete antibody after thawing, and thus demonstrate the feasibility of B cell immune monitoring using cryopreserved PBMC. View Full-Text
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Fecher, P.; Caspell, R.; Naeem, V.; Karulin, A.Y.; Kuerten, S.; Lehmann, P.V. B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody. Cells 2018, 7, 50.
Fecher P, Caspell R, Naeem V, Karulin AY, Kuerten S, Lehmann PV. B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody. Cells. 2018; 7(6):50.Chicago/Turabian Style
Fecher, Philipp; Caspell, Richard; Naeem, Villian; Karulin, Alexey Y.; Kuerten, Stefanie; Lehmann, Paul V. 2018. "B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody." Cells 7, no. 6: 50.
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