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10.3390/cells13110938
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Article

KR158 Spheres Harboring Slow-Cycling Cells Recapitulate High-Grade Glioma Features in an Immunocompetent System

by
Avirup Chakraborty
1,2,
Changlin Yang
1,2,
Jesse L. Kresak
3,
Aryeh J. Silver
1,
Diana Feier
1,
Guimei Tian
4,
Michael Andrews
5,
Olusegun O. Sobanjo
1,
Ethan D. Hodge
1,
Mia K. Engelbart
1,
Jianping Huang
1,2,
Jeffrey K. Harrison
2,6,
Matthew R. Sarkisian
2,7,
Duane A. Mitchell
1,2 and
Loic P. Deleyrolle
1,2,7,*
1
Adam Michael Rosen Neuro-Oncology Laboratories, Department of Neurosurgery, University of Florida, Gainesville, FL 32608, USA
2
Preston A. Wells Jr. Center for Brain Tumor Therapy, University of Florida, Gainesville, FL 32608, USA
3
Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA
4
Department of Surgery, University of Florida, Gainesville, FL 32610, USA
5
College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL 33314, USA
6
Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL 32603, USA
7
Department of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA
*
Author to whom correspondence should be addressed.
Cells 2024, 13(11), 938; https://doi.org/10.3390/cells13110938
Submission received: 4 March 2024 / Revised: 20 May 2024 / Accepted: 24 May 2024 / Published: 29 May 2024
(This article belongs to the Special Issue The Pivotal Role of Tumor Stem Cells in Glioblastoma)
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Abstract

Glioblastoma (GBM) poses a significant challenge in clinical oncology due to its aggressive nature, heterogeneity, and resistance to therapies. Cancer stem cells (CSCs) play a critical role in GBM, particularly in treatment resistance and tumor relapse, emphasizing the need to comprehend the mechanisms regulating these cells. Also, their multifaceted contributions to the tumor microenvironment (TME) underline their significance, driven by their unique properties. This study aimed to characterize glioblastoma stem cells (GSCs), specifically slow-cycling cells (SCCs), in an immunocompetent murine GBM model to explore their similarities with their human counterparts. Using the KR158 mouse model, we confirmed that SCCs isolated from this model exhibited key traits and functional properties akin to human SCCs. KR158 murine SCCs, expanded in the gliomasphere assay, demonstrated sphere forming ability, self-renewing capacity, positive tumorigenicity, enhanced stemness and resistance to chemotherapy. Together, our findings validate the KR158 murine model as a framework to investigate GSCs and SCCs in GBM pathology, and explore specifically the SCC–immune system communications, understand their role in disease progression, and evaluate the effect of therapeutic strategies targeting these specific connections.
Keywords: glioblastoma; cancer stem cells; slow-cycling cells; tumor heterogeneity; immunocompetent murine model glioblastoma; cancer stem cells; slow-cycling cells; tumor heterogeneity; immunocompetent murine model
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MDPI and ACS Style

Chakraborty, A.; Yang, C.; Kresak, J.L.; Silver, A.J.; Feier, D.; Tian, G.; Andrews, M.; Sobanjo, O.O.; Hodge, E.D.; Engelbart, M.K.; et al. KR158 Spheres Harboring Slow-Cycling Cells Recapitulate High-Grade Glioma Features in an Immunocompetent System. Cells 2024, 13, 938. https://doi.org/10.3390/cells13110938

AMA Style

Chakraborty A, Yang C, Kresak JL, Silver AJ, Feier D, Tian G, Andrews M, Sobanjo OO, Hodge ED, Engelbart MK, et al. KR158 Spheres Harboring Slow-Cycling Cells Recapitulate High-Grade Glioma Features in an Immunocompetent System. Cells. 2024; 13(11):938. https://doi.org/10.3390/cells13110938

Chicago/Turabian Style

Chakraborty, Avirup, Changlin Yang, Jesse L. Kresak, Aryeh J. Silver, Diana Feier, Guimei Tian, Michael Andrews, Olusegun O. Sobanjo, Ethan D. Hodge, Mia K. Engelbart, and et al. 2024. "KR158 Spheres Harboring Slow-Cycling Cells Recapitulate High-Grade Glioma Features in an Immunocompetent System" Cells 13, no. 11: 938. https://doi.org/10.3390/cells13110938

APA Style

Chakraborty, A., Yang, C., Kresak, J. L., Silver, A. J., Feier, D., Tian, G., Andrews, M., Sobanjo, O. O., Hodge, E. D., Engelbart, M. K., Huang, J., Harrison, J. K., Sarkisian, M. R., Mitchell, D. A., & Deleyrolle, L. P. (2024). KR158 Spheres Harboring Slow-Cycling Cells Recapitulate High-Grade Glioma Features in an Immunocompetent System. Cells, 13(11), 938. https://doi.org/10.3390/cells13110938

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