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Depletion of the Origin Recognition Complex Subunits Delays Aging in Budding Yeast

Department of Biology, Institute of Biology and Biotechnology, University of Rzeszów, 35-601 Rzeszów, Poland
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland
The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 30-239 Krakow, Poland
Institute of Agricultural Sciences, Land Management and Environmental Protection, University of Rzeszow, 35-601 Rzeszów, Poland
Authors to whom correspondence should be addressed.
Academic Editor: Milena Georgieva
Cells 2022, 11(8), 1252;
Received: 2 March 2022 / Revised: 31 March 2022 / Accepted: 5 April 2022 / Published: 7 April 2022
(This article belongs to the Special Issue Yeast as a Model in Aging Research)
Precise DNA replication is pivotal for ensuring the accurate inheritance of genetic information. To avoid genetic instability, each DNA fragment needs to be amplified only once per cell cycle. DNA replication in eukaryotes starts with the binding of the origin recognition complex (ORC) to the origins of DNA replication. The genes encoding ORC subunits have been conserved across eukaryotic evolution and are essential for the initiation of DNA replication. In this study, we conducted an extensive physiological and aging-dependent analysis of heterozygous cells lacking one copy of ORC genes in the BY4743 background. Cells with only one copy of the ORC genes showed a significant decrease in the level of ORC mRNA, a delay in the G1 phase of the cell cycle, and an extended doubling time. Here, we also show that the reducing the levels of Orc1-6 proteins significantly extends both the budding and average chronological lifespans. Heterozygous ORC/orcΔ and wild-type diploid cells easily undergo haploidization during chronological aging. This ploidy shift might be related to nutrient starvation or the inability to survive under stress conditions. A Raman spectroscopy analysis helped us to strengthen the hypothesis of the importance of lipid metabolism and homeostasis in aging. View Full-Text
Keywords: aging; cell cycle; lifespan; ORC; replication; yeast aging; cell cycle; lifespan; ORC; replication; yeast
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MDPI and ACS Style

Stępień, K.; Skoneczna, A.; Kula-Maximenko, M.; Jurczyk, Ł.; Mołoń, M. Depletion of the Origin Recognition Complex Subunits Delays Aging in Budding Yeast. Cells 2022, 11, 1252.

AMA Style

Stępień K, Skoneczna A, Kula-Maximenko M, Jurczyk Ł, Mołoń M. Depletion of the Origin Recognition Complex Subunits Delays Aging in Budding Yeast. Cells. 2022; 11(8):1252.

Chicago/Turabian Style

Stępień, Karolina, Adrianna Skoneczna, Monika Kula-Maximenko, Łukasz Jurczyk, and Mateusz Mołoń. 2022. "Depletion of the Origin Recognition Complex Subunits Delays Aging in Budding Yeast" Cells 11, no. 8: 1252.

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