Search Results (5,429)

Over the past several decades, the gut microbiome (GM) has been the focus of extensive investigation. In recent years, major discoveries such as the role of maternal breastfeeding in infant GM development and mode of delivery on infant GM health have expanded scientific knowledge on this topic. As this is a rapidly expanding field of research, substantial work remains to further elucidate and integrate the existing evidence on its role in allergic response and immunological development. This comprehensive review will examine the latest discoveries in GM research and its role in the development of food allergies across the lifespan. Examining the existing literature may identify knowledge gaps regarding precise mechanisms through which the development of GM influences the maturation of the immune system. Given the abundance of the literature, we conducted a database search for articles published within the past 10 years. A total of 56 original research articles were retrieved, analyzed, and included in our review. This review article aims to integrate the current evidence on understanding how the development of GM impacts the immune system and food allergy response throughout the lifespan. We aim to uncover microbial mechanisms of allergy response, host and microbe interactions, and opportunities for therapeutic intervention. Additionally, we aim to reveal gaps in the current knowledge of the GM’s influence on allergy development, offering directions for future research. Full article

This paper proposes a co-design methodology for the power and control stages of a bidirectional battery charger/discharger based on a boost converter topology. The approach ensures safe operation by limiting the battery current derivative, preventing abrupt transients that could degrade battery lifespan. The control strategy combines a cascade structure with an inner sliding mode current controller (for robustness and fast response) and an outer adaptive PI voltage loop (to regulate the DC-link voltage under varying load conditions). Additionally, the design constrains the switching frequency to reduce power losses. Experimental validation on a prototype converter demonstrates the effectiveness of the co-design framework, showing precise current/voltage regulation, adherence to switching frequency limits, and compliance with battery charging/discharging requirements. The results highlight the methodology’s potential to enhance efficiency and reliability in energy storage systems. The dynamic restrictions, overshoot lower than 5%, settling time shorter than 5 ms, and a battery current limitation less than 50 A/ms were always met with SMC and, in some cases, with the PI controller, but the results with SMC were always better: lower overshoot, shorter settling time, and greater restriction on the derivative of the battery current. In addition, the SMC system was 2.5–5.0% more efficient than the PI controller. Full article

Despite broad agreement on sensorimotor foundations of cognition, existing integrative models are not user-friendly to those who most need them—parents, caregivers, teachers, and clinical practitioners. This review addresses that gap by proposing Meaning-Events (M-Es) as sensorimotor–cognitive coordination units that structure meaning from infancy through adulthood. Drawing on joint attention research, embodied cognition, and dynamic skill theory, this integrative model demonstrates how sensorimotor processes—gaze coordination, affective timing/synchrony, bodily orientation, eye–hand coordination, and goal-directed action—organize cognitive structures of increasingly abstract meaning-making. Meaning-Events are shown as the smallest analyzable units that integrate sensorimotor experience with cognition, providing (1) developmental continuity for embodied action giving rise to coherent thought, purpose, and identity; (2) reciprocal perspectives informing impacts of dyadic behavioral interactions; and (3) an analytical and synthetic tool providing visible, measurable differentiation and integration of behaviors over time. Integration of Fischer’s dynamic skill theory with Erikson’s psychosocial theory illustrates applications in clinical and educational contexts. Rather than viewing sensorimotor experience as an early precursor superseded by symbolic cognition, the Meaning-Event model positions these sensorimotor–cognitive coordination units as constitutive of meaning at all developmental levels. Full article

Human lifespan is increasing in parallel with the development levels of societies. Consequently, the number of elderly individuals worldwide is also rising day by day. One of the most significant risks these individuals face is falling. In this study, fall and daily activity data were collected from different home environments using a continuous-wave (CW) radar. Micro-Doppler signatures were generated from 700 data samples obtained from 10 individuals. Furthermore, the dataset was expanded by doubling the number of spectrogram images through data augmentation. The YOLO architecture, generally used in vision-based studies for object detection and tracking, was preferred for radar-based fall and activity detection. Classifications were performed with different YOLO structures, and comparative results are presented. At this stage, binary (fall/non-fall) and multi-class (seven different classes) classifications were carried out, achieving 100% accuracy for binary classification and 88.02% for multi-class classification. Additionally, the generalizability of the proposed architecture is demonstrated using the Leave-One-Subject-Out (LOSO) approach on the collected data and through the analysis of a public dataset. These results demonstrate the applicability of YOLO architectures in radar-based fall detection studies. Full article

This study conducts a cradle-to-gate Environmental Life Cycle Assessment (E-LCA) of tea production in Sri Lanka, comparing smallholder and estate-owned plantations processed by Orthodox and Crush–Tear–Curl (CTC) methods. Unlike most tea LCA studies that treat cultivation as a single undifferentiated phase, this work explicitly incorporates the perennial nature of tea by using a modular life cycle framework that separates the agronomic stages alongside factory processing up to the packed-tea gate. This approach allows a more precise allocation of long-term environmental burdens over the entire productive lifespan of the tea plant, addressing a methodological gap in the literature. Four production scenarios were evaluated: Smallholder-Orthodox, Smallholder-CTC, Estate-Orthodox, and Estate-CTC, with the functional unit set to 1 tonne of processed tea. Primary data were gathered through structured surveys of 30 plantations (25 smallholders, 5 estates) and 5 tea factories, supplemented by secondary data from Ecoinvent v3.11 and national statistics. The CML-IA Baseline method in SimaPro v9.5 was applied to characterize impacts across eight impact categories: global warming potential (GWP), abiotic element depletion, fossil fuel depletion, acidification, human toxicity, terrestrial ecotoxicity, freshwater ecotoxicity, and eutrophication. Results indicate that Smallholder-Orthodox systems have the highest GWP (3304 kg CO₂ eq per tonne), whereas Estate-CTC systems show a lower GWP (2894.87 kg CO₂ eq). Acidification potential ranges from 47.21 kg SO₂ eq for Smallholder-Orthodox to 41.25 kg SO₂ eq for Estate-CTC. Overall, the findings suggest that the scale of plantation management has a greater impact on environmental performance than processing technology, highlighting the need to focus sustainable practices on the cultivation stage, exactly where the perennial crop modeling approach used here provides the greatest analytical benefit. Full article

Background/Objectives: Physical activity behaviours are established early in life and tend to track across developmental stages. However, the role of continuity of sport participation across multiple developmental periods in shaping current physical activity levels remains insufficiently understood. This study aimed to examine the association between continuity of sport participation across developmental stages and current physical activity levels in university students, and to assess whether this association followed a graded pattern and differed by sex. Methods: A cross-sectional study was conducted among 796 fourth-year undergraduate students enrolled in a Primary School Education degree program at the University of Turin. Data were collected using an anonymous online survey. Current physical activity was assessed using the International Physical Activity Questionnaire—Short Form (IPAQ-SF) and categorised as non-active, sufficiently active, or active. Sport participation across six developmental stages was retrospectively assessed and summarised into a three-level continuity variable (discontinuous, intermediate, continuous). Associations were examined using chi-square tests and ordinal logistic regression models adjusted for sex, age, and body mass index (BMI). Predicted probabilities were estimated to aid interpretation. Results: Continuity of sport participation was significantly associated with current physical activity levels (χ²(6) = 67.55, p < 0.001), with a graded pattern evident. In adjusted models, discontinuous participation (OR = 0.24, 95% CI 0.14–0.39) and intermediate participation (OR = 0.62, 95% CI 0.46–0.82) were associated with lower odds of belonging to higher physical activity categories than continuous participation. Predicted probabilities showed a clear dose–response pattern, with progressively higher likelihoods of being active as continuity increased. This pattern was consistent across sexes, although males exhibited higher overall activity levels. Conclusions: Greater continuity of sport participation across developmental stages is associated with higher current physical activity levels. Promoting sustained engagement in sport may support the development of active lifestyles across the lifespan. Full article

Background: Behavioural therapy (BT), including Comprehensive Behavioural Intervention for Tics (CBIT), is an evidence-based first-line treatment for patients with tic disorders. However, access remains limited due to a shortage of trained providers, geographical barriers, costs, and high treatment burden for patients and families. Rapid advances in digital health technologies including telemedicine, web-based treatment platforms, and mobile applications offer new opportunities to expand access to BT for individuals with tic disorders across the lifespan. Methods: For the purpose of this narrative review, we conducted a literature search in PubMed, Europe PMC, and the Cochrane Library to identify relevant studies investigating the effectiveness of digital health treatment approaches in tic disorders. Results: A total of 16 original studies were included. Although the available evidence remains limited and heterogeneous, existing studies suggest that emerging technologies for delivering behavioural interventions for tic disorders, including telehealth-based CBIT, digital therapy platforms, and app-supported habit reversal training (HRT), are feasible, cost-effective, user-friendly, flexible, and safe. These approaches also appear effective for symptom monitoring and personalized treatment support in both pediatric and adult populations. Conclusions: Recent technological advances have the potential to reduce the treatment gap in tic disorders, provided that these approaches are implemented within rigorous, evidence-based, and ethically grounded frameworks. Full article

Arginase 1 (ARG1) deficiency (ARG1-D) is a rare genetic disorder due to loss of ARG1, the final enzyme in the urea cycle. ARG1-D hepatocytes are impaired in converting arginine into urea, resulting in elevated peripheral arginine and ammonia, which leads to progressive neurological symptoms. Current therapeutic strategies mainly focus on managing plasma arginine and ammonia level, but long-term outcomes remain poor. While no approved treatment specific for ARG1-D is available in the United States, a recombinant protein-based enzyme replacement therapy is available in Europe. Recently, extracellular vesicles (EVs) are emerging as a powerful therapeutic vehicle. By using Capricor’s StealthX^TM platform, EVs were engineered to express human ARG1 on their surface or encapsulated within. Regardless of their localization on the EV membrane, nanograms of ARG1 carried by EVs were biologically active and able to convert arginine into urea as potent as micrograms of human recombinant ARG1 (rHuArg1). Furthermore, ARG1-encapsulating EVs (STX-Arg1-in) were able to deliver ARG1 intracellularly but not EVs carrying ARG1 on their surface or rHuArg1. STX-Arg1-in EVs were further evaluated in a series of in vivo studies, and the results showed that STX-Arg1-in EVs were non-toxic and able to restore arginase activities in the liver of Arg1^−/− mice, which led to a lowered plasma arginine concentration similar to that in wild-type mice. Most importantly, Arg1-in EVs expanded the lifespan of the lethal neonatal Arg1 deficiency mouse model. Taken together, our data suggested StealthX^TM-engineered STX-Arg1-in EVs have a better safety profile due to the extremely low dosage and have great potential as a novel enzyme replacement strategy for patients suffering from ARG1-D. Significance statement: Intracellular delivery of recombinant protein and improved llifespanare endpoints of successful enzyme replacement therapy for the treatment of ARG1-D. Using the StealthX platform, a fully functional ARG1 enzyme was engineered to be carried inside of the extracellular vesicles, which allowed for the intracellular delivery of ARG1 protein in vitro and in vivo, with an improvement of lifespan in a lethal neonatal mouse model of Arg1 deficiency. More importantly, no toxicity was observed, and efficacy was achieved with a low dose, setting the base for an improved therapeutic approach. Full article

Artificial Intelligence of Things (AIoT) technologies shifted the structure of production systems, enabling the development of more intelligent, connected and sustainable manufacturing environments. However, some industrial sectors, such as aerospace manufacturing industry, fell behind in the adoption of these new technologies, mainly because of the high safety standards, strict reliability requirements and long lifespan of aircraft components. Due to low production volumes and complex manufacturing processes, this sector relies heavily on weakly automated legacy machines and production systems. This article proposes a methodology to ease the integration of AIoT technologies for retrofitting legacy industrial equipment in the aeronautical domain in order to achieve the requirements of modern industrial production systems, enabling the development of more flexible, efficient and interconnected manufacturing environments. The proposed methodology is validated through a case study where the Smart Retrofitting of a legacy aeronautical industrial machine is carried out. The case study focuses on the development of an AIoT-based architecture to implement a predictive maintenance system through vibration and infrared thermography monitoring. A three layer architecture is proposed based on Edge/Fog/Cloud Computing paradigms. A hybrid communication architecture is used, combining wired technologies for critical real-time control tasks and wireless technologies for enhanced flexibility and scalability. The results demonstrate the viability of the proposed methodology for retrofitting legacy aircraft manufacturing systems. Full article

Trained immunity refers to the enduring functional reprogramming of innate immune cells after particular stimuli, driven by epigenetic and metabolic alterations that augment non-specific responses upon subsequent exposure. Neutrophils and monocytes/macrophages, as essential innate effectors, are crucial for the induction and control of trained immunity, which is the primary emphasis of this review. Neutrophils, the predominant circulating leukocytes, were historically considered incapable of memory owing to their brief lifespan. Emerging evidence indicates that trained immunity functions at the bone marrow progenitor level, influencing granulopoiesis to produce neutrophils with lasting functional modifications. This research offers new insights into neutrophil functions in infection, cancer, and inflammation. Monocytes and macrophages, characterized by phenotypic plasticity and tissue residence, function as conventional models of trained immunity. They experience direct peripheral reprogramming or emerge as primed descendants of trained bone marrow precursors, performing pro-inflammatory or reparative roles in malignancies, infections, and ischemia lesions. This study comprehensively outlines the regulatory mechanisms of trained immunity in these cells, clarifies their functions in various clinical situations, and examines therapeutic applications. Comprehending these pathways is crucial for elucidating the cellular foundation of innate immunological memory, uncovering its multiple functions in disease, and guiding innovative therapeutics aimed at granulopoiesis and monocyte-macrophage polarization. Full article

Kidney development in the first 1000 days of life is vulnerable to numerous prenatal, perinatal, and congenital factors. This review aims to analyze the main determinants of early kidney development and to highlight the role of pediatricians in identifying at-risk infants and implementing preventive strategies to reduce the risk of chronic kidney disease (CKD). For at-risk newborns, early assessment of kidney size and function is essential for the timely detection of functional decline. Key risk factors include prenatal exposures, perinatal complications, genetic conditions, and postnatal factors. Early, tailored nephrological follow-up is crucial for preventing CKD and its complications. Determining optimal monitoring intervals through clinical, laboratory, and ultrasound evaluations enables risk stratification, ensuring closer surveillance for the most vulnerable infants during this critical window. This review integrates evidence from experimental, epidemiological, and clinical studies and highlights the importance of early-life interventions in shaping renal health across the lifespan. Full article

Wide-bandgap power devices, particularly silicon carbide (SiC) MOSFETs, have seen widespread adoption in electric vehicle (EV) motor drive systems due to their superior switching characteristics, including high switching speeds and high switching frequencies. However, these advantages exacerbate motor terminal overvoltage, with peaks reaching twice the inverter output voltage, causing insulation breakdown in windings and bearing electro-corrosion, which shorten motor lifespan. Traditional overvoltage prediction methods, such as distributed parameter models or detailed ladder network approaches, require extensive system parameters and involve high computational loads, while simplified models lack generality. To address these issues, this paper proposes a simplified prediction method based on a lumped ladder network model combined with frequency scanning. The approach uses impedance analysis to identify anti-resonance frequencies, enabling direct estimation of overvoltage amplitudes without prior knowledge of cable or motor specifics. Experimental validation on a SiC-based drive system demonstrates prediction errors below 10% and a reduction in computational time compared to conventional methods. Full article

Background: Crocodilians rarely develop cancer despite long lifespans and continuous exposure to environmental carcinogens, suggesting robust natural anti-tumor defense mechanisms. Methods: We investigated the anti-cancer activity of sera derived from the phylogenetically related species—alligators, crocodiles, and chickens, and studied their underlying immune mechanisms. The anti-tumor activity of alligator serum was tested in murine models of melanoma and lymphoma. Results: Alligator serum (AS) and its (NH₄)₂SO₄-precipitated fraction (ASa) showed rapid and potent cytotoxicity toward multiple murine and human cancer cell lines while sparing non-malignant human cells. Importantly, ASa attenuated melanoma and lymphoma tumor growth in mice. Electrophysiological analyses in PN71 cancer cells treated with ASa revealed rapid membrane depolarization and formation of high-conductance pores consistent with Complement-mediated membrane attack complex (MAC) activity. Proteomic analyses identified the Complement component C5 as a major protein enriched in active fractions, implicating the Complement system in cancer cell killing. Based on phylogenetic similarity of C5, crocodile and chicken sera exhibit alligator-like comparable anti-cancer activity. Mechanistic studies in chicken serum showed that the anti-cancer activity depends on Ca²⁺ and Mg²⁺ ions, terminal Complement components (C5–C8), and IgM antibodies that initiate Complement activation. Immunodepletion of IgM from CSa significantly reduced cytotoxicity, whereas purified chicken IgM activated human Complement to induce cancer cell death. Conclusions: These findings identify a conserved IgM–Complement immune mechanism capable of selectively targeting malignant cells. The evolutionary conservation and cross-species functionality of this pathway highlight its potential as a bio-inspired strategy for developing novel Complement-based cancer immunotherapies. Full article

Apolygus lucorum (Meyer-Dür) is a phytozoophagous crop pest. While the effects of plant-based diets on its development and reproduction have been extensively studied, the combined effects of plant- and prey-based diets on these traits remain poorly understood. This study systematically evaluated the effects of plant-only, prey-only, and mixed plant–prey diets on A. lucorum nymphal survival and development, as well as adult longevity and fecundity, under controlled laboratory conditions. The results demonstrate that diet composition significantly affected nymphal survival and developmental progression. Nymphs fed exclusively on prey (Aphis gossypii Glover or Bemisia tabaci (Gennadius) nymphs) failed to complete juvenile development. Although a diet of Helicoverpa armigera (Hübner) eggs alone enabled some individuals to reach adulthood, survival rates were significantly lower than those in mixed-diet treatments. Mixed feeding markedly improved nymphal survival, with the highest rates observed in groups fed green beans + H. armigera eggs and cotton leaves + B. tabaci nymph combinations (both 64.45%). The developmental duration was also influenced. Mixed diets, particularly green beans + H. armigera eggs, significantly shortened each instar and the total developmental time (11.04 ± 0.17 d), whereas a diet of cotton leaves alone prolonged development (19.45 ± 0.24 d). Adult longevity and reproductive output were likewise diet-dependent. The longest lifespans were recorded in adults fed green beans alone or green beans + H. armigera eggs, while the shortest lifespan was observed for those fed only cotton leaves. Successful oviposition was only achieved following four dietary treatments: green beans alone, green beans + H. armigera eggs, H. armigera eggs alone, and cotton leaves + H. armigera eggs. Among these, the green bean + H. armigera egg diet yielded the best reproductive performance, featuring the shortest pre-oviposition period (5.82 ± 0.60 d), the longest oviposition period (19.41 ± 1.68 d), and the highest mean fecundity per female (238.35 ± 25.51 eggs). This underscores the reproductive advantage of a mixed plant–prey diet. This study clarifies how dietary conditions shape the survival, development, and reproduction of A. lucorum, highlighting its strong reliance on nutritional quality for key life-history traits. These findings offer valuable insights into the ecological adaptations underlying the feeding behavior of this insect. Full article

Background: Autism Spectrum Disorder (ASD) is a heterogeneous neurodevelopmental condition for which non-pharmacological interventions remain the primary therapeutic approach. Although research output in this field has increased substantially, a comprehensive synthesis of its developmental trajectory and emerging directions is still lacking. Methods: We conducted a bibliometric analysis of publications on non-pharmacological interventions for ASD indexed in the Web of Science Core Collection between 2001 and 2025. Knowledge structures, research hotspots, and temporal trends were visualized and analyzed using CiteSpace. Results: The field has transitioned from an early focus on behavioral interventions in children to a diversified and interdisciplinary research ecosystem spanning the lifespan. Recent growth has been driven by the integration of neuroscience-based approaches, particularly neuromodulation techniques, alongside continued refinement of behavioral, sensorimotor, and complementary therapies. Increasing attention has been paid to individual heterogeneity, methodological rigor, and mechanism-oriented research. Current frontiers emphasize multimodal intervention strategies, neural plasticity-based mechanisms, and the development of personalized precision intervention frameworks. Conclusions: This bibliometric analysis delineates the intellectual evolution of non-pharmacological intervention research for ASD and identifies key research gaps, particularly the need for longitudinal and pragmatic studies targeting individualized treatment response. The findings provide an evidence-informed overview of current concepts and emerging research directions in non-pharmacological care for ASD, with important implications for future clinical research, intervention design, and strategic research planning. Full article