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Cells
Volume 11
Issue 5
10.3390/cells11050787
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Brief Report

DNA Methyltransferase 3b in Myeloid Cells Does Not Affect the Acute Immune Response in the Airways during Pseudomonas Pneumonia

by
Wanhai Qin
1,*,
Xanthe Brands
1,
Cornelis van’t Veer
1,
Alex F. de Vos
1,
Brendon P. Scicluna
1,2 and
Tom van der Poll
1,3
1
Center of Experimental & Molecular Medicine, Amsterdam University Medical Centers, Location Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
2
Department of Clinical Epidemiology, Biostatistics, and Bioinformatics, Amsterdam University Medical Centers, Location Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
3
Division of Infectious Diseases, Amsterdam University Medical Centers, Location Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Cells 2022, 11(5), 787; https://doi.org/10.3390/cells11050787
Submission received: 29 January 2022 / Revised: 18 February 2022 / Accepted: 23 February 2022 / Published: 24 February 2022
(This article belongs to the Special Issue Cellular Reprogramming during Sepsis and Potential Therapeutic Targets)
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Abstract

DNA methyltransferase 3b (Dnmt3b) has been suggested to play a role in the host immune response during bacterial infection. Neutrophils and other myeloid cells are crucial for lung defense against Pseudomonas (P.) aeruginosa infection. This study aimed to investigate the role of Dnmt3b in neutrophils and myeloid cells during acute pneumonia caused by P. aeruginosa. Neutrophil-specific (Dnmt3bfl/flMrp8Cre) or myeloid cell-specific (Dnmt3bfl/flLysMCre) Dnmt3b-deficient mice and littermate control mice were infected with P. aeruginosa PAK via the airways. Bacteria burdens, neutrophil recruitment, and activation (CD11b expression, myeloperoxidase, and elastase levels), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) were measured in bronchoalveolar lavage fluid (BALF) at 6 and 24 h after infection. Our data showed that the bacterial loads and neutrophil recruitment and activation did not differ in BALF obtained from neutrophil-specific Dnmt3b-deficient and control mice, whilst BALF IL-6 and TNF levels were lower in the former group at 24 but not at 6 h after infection. None of the host response parameters measured differed between myeloid cell-specific Dnmt3b-deficient and control mice. In conclusion, dnmt3b deficiency in neutrophils or myeloid cells does not affect acute immune responses in the airways during Pseudomonas pneumonia.
Keywords: Dnmt3b; neutrophils; P. aeruginosa; pneumonia Dnmt3b; neutrophils; P. aeruginosa; pneumonia

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MDPI and ACS Style

Qin, W.; Brands, X.; van’t Veer, C.; de Vos, A.F.; Scicluna, B.P.; van der Poll, T. DNA Methyltransferase 3b in Myeloid Cells Does Not Affect the Acute Immune Response in the Airways during Pseudomonas Pneumonia. Cells 2022, 11, 787. https://doi.org/10.3390/cells11050787

AMA Style

Qin W, Brands X, van’t Veer C, de Vos AF, Scicluna BP, van der Poll T. DNA Methyltransferase 3b in Myeloid Cells Does Not Affect the Acute Immune Response in the Airways during Pseudomonas Pneumonia. Cells. 2022; 11(5):787. https://doi.org/10.3390/cells11050787

Chicago/Turabian Style

Qin, Wanhai, Xanthe Brands, Cornelis van’t Veer, Alex F. de Vos, Brendon P. Scicluna, and Tom van der Poll. 2022. "DNA Methyltransferase 3b in Myeloid Cells Does Not Affect the Acute Immune Response in the Airways during Pseudomonas Pneumonia" Cells 11, no. 5: 787. https://doi.org/10.3390/cells11050787

APA Style

Qin, W., Brands, X., van’t Veer, C., de Vos, A. F., Scicluna, B. P., & van der Poll, T. (2022). DNA Methyltransferase 3b in Myeloid Cells Does Not Affect the Acute Immune Response in the Airways during Pseudomonas Pneumonia. Cells, 11(5), 787. https://doi.org/10.3390/cells11050787

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