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Article

The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators

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Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium
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VIB Center for Plant Systems Biology, 9052 Ghent, Belgium
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Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UK
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Integrated Molecular Plant Physiology Research, Biology Department, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium
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Institute of Science and Technology (IST) Austria, 3400 Klosterneuburg, Austria
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The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China
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Plant Biochemistry & Biotechnology Lab, Department of Agriculture, Hellenic Mediterranean University, Stavromenos, 71410 Heraklion, Crete, Greece
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Institute for Life and Earth Sciences, School of Energy, Geosciences, Infrastructure and Society, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK
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Centre for Plant Integrative Biology, University of Nottingham, Loughborough LE12 5RD, UK
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Author to whom correspondence should be addressed.
Academic Editor: Suleyman Allakhverdiev
Cells 2021, 10(7), 1665; https://doi.org/10.3390/cells10071665
Received: 15 April 2021 / Revised: 15 June 2021 / Accepted: 28 June 2021 / Published: 2 July 2021
(This article belongs to the Special Issue Protein Phosphorylation and Cell Signaling in Plants)
Auxin plays a dual role in growth regulation and, depending on the tissue and concentration of the hormone, it can either promote or inhibit division and expansion processes in plants. Recent studies have revealed that, beyond transcriptional reprogramming, alternative auxin-controlled mechanisms regulate root growth. Here, we explored the impact of different concentrations of the synthetic auxin NAA that establish growth-promoting and -repressing conditions on the root tip proteome and phosphoproteome, generating a unique resource. From the phosphoproteome data, we pinpointed (novel) growth regulators, such as the RALF34-THE1 module. Our results, together with previously published studies, suggest that auxin, H+-ATPases, cell wall modifications and cell wall sensing receptor-like kinases are tightly embedded in a pathway regulating cell elongation. Furthermore, our study assigned a novel role to MKK2 as a regulator of primary root growth and a (potential) regulator of auxin biosynthesis and signalling, and suggests the importance of the MKK2 Thr31 phosphorylation site for growth regulation in the Arabidopsis root tip. View Full-Text
Keywords: primary root; (phospho)proteomics; auxin; (receptor) kinase primary root; (phospho)proteomics; auxin; (receptor) kinase
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MDPI and ACS Style

Nikonorova, N.; Murphy, E.; Fonseca de Lima, C.F.; Zhu, S.; van de Cotte, B.; Vu, L.D.; Balcerowicz, D.; Li, L.; Kong, X.; De Rop, G.; Beeckman, T.; Friml, J.; Vissenberg, K.; Morris, P.C.; Ding, Z.; De Smet, I. The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators. Cells 2021, 10, 1665. https://doi.org/10.3390/cells10071665

AMA Style

Nikonorova N, Murphy E, Fonseca de Lima CF, Zhu S, van de Cotte B, Vu LD, Balcerowicz D, Li L, Kong X, De Rop G, Beeckman T, Friml J, Vissenberg K, Morris PC, Ding Z, De Smet I. The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators. Cells. 2021; 10(7):1665. https://doi.org/10.3390/cells10071665

Chicago/Turabian Style

Nikonorova, Natalia, Evan Murphy, Cassio F. Fonseca de Lima, Shanshuo Zhu, Brigitte van de Cotte, Lam D. Vu, Daria Balcerowicz, Lanxin Li, Xiangpei Kong, Gieljan De Rop, Tom Beeckman, Jiří Friml, Kris Vissenberg, Peter C. Morris, Zhaojun Ding, and Ive De Smet. 2021. "The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators" Cells 10, no. 7: 1665. https://doi.org/10.3390/cells10071665

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