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Article

The HSP90 Inhibitor, AUY-922, Protects and Repairs Human Lung Microvascular Endothelial Cells from Hydrochloric Acid-Induced Endothelial Barrier Dysfunction

1
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
2
School of Medical Diagnostic & Translational Sciences, College of Health Sciences, Old Dominion University, Norfolk, VA 23508, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Konstantin G Birukov and Alexander E. Kalyuzhny
Cells 2021, 10(6), 1489; https://doi.org/10.3390/cells10061489
Received: 6 April 2021 / Revised: 2 June 2021 / Accepted: 10 June 2021 / Published: 13 June 2021
(This article belongs to the Collection The Endothelial Cell in Lung Inflammation)
Exposure to hydrochloric acid (HCl) leads acutely to asthma-like symptoms, acute respiratory distress syndrome (ARDS), including compromised alveolo-capillary barrier, and respiratory failure. To better understand the direct effects of HCl on pulmonary endothelial function, we studied the characteristics of HCl-induced endothelial barrier dysfunction in primary cultures of human lung microvascular endothelial cells (HLMVEC), defined the involved molecular pathways, and tested the potentially beneficial effects of Heat Shock Protein 90 (HSP90) inhibitors. HCl impaired barrier function in a time- and concentration-dependent manner and was associated with activation of Protein Kinase B (AKT), Ras homolog family member A (RhoA) and myosin light chain 2 (MLC2), as well as loss of plasmalemmal VE-cadherin, rearrangement of cortical actin, and appearance of inter-endothelial gaps. Pre-treatment or post-treatment of HLMVEC with AUY-922, a third-generation HSP90 inhibitor, prevented and restored HCl-induced endothelial barrier dysfunction. AUY-922 increased the expression of HSP70 and inhibited the activation (phosphorylation) of extracellular-signal regulated kinase (ERK) and AKT. AUY-922 also prevented the HCl-induced activation of RhoA and MLC2 and the internalization of plasmalemmal VE-cadherin. We conclude that, by increasing the expression of cytoprotective proteins, interfering with actomyosin contractility, and enhancing the expression of junction proteins, inhibition of HSP90 may represent a useful approach for the management of HCl-induced endothelial dysfunction and acute lung injury. View Full-Text
Keywords: acidosis; hydrochloric acid; HCl; endothelial dysfunction; heat shock protein; HSP90 inhibition; AUY-922; acute lung injury acidosis; hydrochloric acid; HCl; endothelial dysfunction; heat shock protein; HSP90 inhibition; AUY-922; acute lung injury
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MDPI and ACS Style

Colunga Biancatelli, R.M.L.; Solopov, P.; Gregory, B.; Catravas, J.D. The HSP90 Inhibitor, AUY-922, Protects and Repairs Human Lung Microvascular Endothelial Cells from Hydrochloric Acid-Induced Endothelial Barrier Dysfunction. Cells 2021, 10, 1489. https://doi.org/10.3390/cells10061489

AMA Style

Colunga Biancatelli RML, Solopov P, Gregory B, Catravas JD. The HSP90 Inhibitor, AUY-922, Protects and Repairs Human Lung Microvascular Endothelial Cells from Hydrochloric Acid-Induced Endothelial Barrier Dysfunction. Cells. 2021; 10(6):1489. https://doi.org/10.3390/cells10061489

Chicago/Turabian Style

Colunga Biancatelli, Ruben M. L., Pavel Solopov, Betsy Gregory, and John D. Catravas. 2021. "The HSP90 Inhibitor, AUY-922, Protects and Repairs Human Lung Microvascular Endothelial Cells from Hydrochloric Acid-Induced Endothelial Barrier Dysfunction" Cells 10, no. 6: 1489. https://doi.org/10.3390/cells10061489

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