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Open AccessArticle

Eosinophil Responses at the Airway Epithelial Barrier during the Early Phase of Influenza a Virus Infection in C57BL/6 Mice

1
Division of Pulmonology, Allergy-Immunology and Sleep, Department of Pediatrics, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
2
Children’s Foundation Research Institute, Memphis, TN 38105, USA
3
Department of Immunology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
4
Department of Microbiology, Immunology and Biochemistry, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Stephane Esnault, Mats W. Johansson and Sameer K. Mathur
Cells 2021, 10(3), 509; https://doi.org/10.3390/cells10030509
Received: 17 December 2020 / Revised: 9 February 2021 / Accepted: 12 February 2021 / Published: 27 February 2021
(This article belongs to the Special Issue Eosinophils beyond IL-5)
Eosinophils, previously considered terminally differentiated effector cells, have multifaceted functions in tissues. We previously found that allergic mice with eosinophil-rich inflammation were protected from severe influenza and discovered specialized antiviral effector functions for eosinophils including promoting cellular immunity during influenza. In this study, we hypothesized that eosinophil responses during the early phase of influenza contribute to host protection. Using in vitro and in vivo models, we found that eosinophils were rapidly and dynamically regulated upon influenza A virus (IAV) exposure to gain migratory capabilities to traffic to lymphoid organs after pulmonary infection. Eosinophils were capable of neutralizing virus upon contact and combinations of eosinophil granule proteins reduced virus infectivity through hemagglutinin inactivation. Bi-directional crosstalk between IAV-exposed epithelial cells and eosinophils occurred after IAV infection and cross-regulation promoted barrier responses to improve antiviral defenses in airway epithelial cells. Direct interactions between eosinophils and airway epithelial cells after IAV infection prevented virus-induced cytopathology in airway epithelial cells in vitro, and eosinophil recipient IAV-infected mice also maintained normal airway epithelial cell morphology. Our data suggest that eosinophils are important in the early phase of IAV infection providing immediate protection to the epithelial barrier until adaptive immune responses are deployed during influenza. View Full-Text
Keywords: adhesion molecules; cell damage; activation; migration adhesion molecules; cell damage; activation; migration
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MDPI and ACS Style

Tiwary, M.; Rooney, R.J.; Liedmann, S.; LeMessurier, K.S.; Samarasinghe, A.E. Eosinophil Responses at the Airway Epithelial Barrier during the Early Phase of Influenza a Virus Infection in C57BL/6 Mice. Cells 2021, 10, 509. https://doi.org/10.3390/cells10030509

AMA Style

Tiwary M, Rooney RJ, Liedmann S, LeMessurier KS, Samarasinghe AE. Eosinophil Responses at the Airway Epithelial Barrier during the Early Phase of Influenza a Virus Infection in C57BL/6 Mice. Cells. 2021; 10(3):509. https://doi.org/10.3390/cells10030509

Chicago/Turabian Style

Tiwary, Meenakshi; Rooney, Robert J.; Liedmann, Swantje; LeMessurier, Kim S.; Samarasinghe, Amali E. 2021. "Eosinophil Responses at the Airway Epithelial Barrier during the Early Phase of Influenza a Virus Infection in C57BL/6 Mice" Cells 10, no. 3: 509. https://doi.org/10.3390/cells10030509

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