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Article

Analysis of Wheat Bread-Making Gene (wbm) Evolution and Occurrence in Triticale Collection Reveal Origin via Interspecific Introgression into Chromosome 7AL

1
Laboratory of Marker-Assisted and Genomic Selection of Plants, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya str. 42, 127550 Moscow, Russia
2
Center of Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Timiryazevskaya str. 49, 127550 Moscow, Russia
*
Author to whom correspondence should be addressed.
Agronomy 2019, 9(12), 854; https://doi.org/10.3390/agronomy9120854
Received: 11 November 2019 / Revised: 28 November 2019 / Accepted: 4 December 2019 / Published: 5 December 2019
(This article belongs to the Special Issue Chromosome Manipulation for Plant Breeding Purposes)
Bread-making quality is a crucial trait for wheat and triticale breeding. Several genes significantly influence these characteristics, including glutenin genes and the wheat bread-making (wbm) gene. World wheat collection screening showed that only a few percent of cultivars carry the valuable wbm variant, providing a useful source for wheat breeding. In contrast, no such analysis has been performed for triticale (wheat (AABB genome) × rye (RR) amphidiploid) collections. Despite the importance of the wbm gene, information about its origin and genomic organization is lacking. Here, using modern genomic resources available for wheat and its relatives, as well as PCR screening, we aimed to examine the evolution of the wbm gene and its appearance in the triticale genotype collection. Bioinformatics analysis revealed that the wheat Chinese Spring genome does not have the wbm gene but instead possesses the orthologous gene, called wbm-like located on chromosome 7A. The analysis of upstream and downstream regions revealed the insertion of LINE1 (Long Interspersed Nuclear Elements) retrotransposons and Mutator DNA transposon in close vicinity to wbm-like. Comparative analysis of the wbm-like region in wheat genotypes and closely related species showed low similarity between the wbm locus and other sequences, suggesting that wbm originated via introgression from unknown species. PCR markers were developed to distinguish wbm and wbm-like sequences, and triticale collection was screened resulting in the detection of three genotypes carrying wbm-specific introgression, providing a useful source for triticale breeding programs. View Full-Text
Keywords: triticale; wheat bread-making gene; introgression; PCR markers triticale; wheat bread-making gene; introgression; PCR markers
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MDPI and ACS Style

Kirov, I.; Pirsikov, A.; Milyukova, N.; Dudnikov, M.; Kolenkov, M.; Gruzdev, I.; Siksin, S.; Khrustaleva, L.; Karlov, G.; Soloviev, A. Analysis of Wheat Bread-Making Gene (wbm) Evolution and Occurrence in Triticale Collection Reveal Origin via Interspecific Introgression into Chromosome 7AL. Agronomy 2019, 9, 854. https://doi.org/10.3390/agronomy9120854

AMA Style

Kirov I, Pirsikov A, Milyukova N, Dudnikov M, Kolenkov M, Gruzdev I, Siksin S, Khrustaleva L, Karlov G, Soloviev A. Analysis of Wheat Bread-Making Gene (wbm) Evolution and Occurrence in Triticale Collection Reveal Origin via Interspecific Introgression into Chromosome 7AL. Agronomy. 2019; 9(12):854. https://doi.org/10.3390/agronomy9120854

Chicago/Turabian Style

Kirov, Ilya, Andrey Pirsikov, Natalia Milyukova, Maxim Dudnikov, Maxim Kolenkov, Ivan Gruzdev, Stanislav Siksin, Ludmila Khrustaleva, Gennady Karlov, and Alexander Soloviev. 2019. "Analysis of Wheat Bread-Making Gene (wbm) Evolution and Occurrence in Triticale Collection Reveal Origin via Interspecific Introgression into Chromosome 7AL" Agronomy 9, no. 12: 854. https://doi.org/10.3390/agronomy9120854

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