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Potato Germplasm Enhancement Enters the Genomics Era
Open AccessArticle

Transcriptome Profiles of Contrasting Potato (Solanum tuberosum L.) Genotypes Under Water Stress

1
National Institute for Agriculture Research (INIA), La Platina, Casilla 439-3, Correo Central, Santiago, Chile
2
Centro de Biotecnología Vegetal, Universidad Andres Bello, República 217, Santiago, Chile
3
Universidad Estatal de O’Higgins, Av. Cachapoal N 90, Rancagua, Chile
4
Agriculture and Agri-Food Canada, Fredericton Research and Development Centre, P.O. Box 20280, 850 Lincoln Rd., Fredericton, NB E3B 4Z7, Canada
*
Authors to whom correspondence should be addressed.
Agronomy 2019, 9(12), 848; https://doi.org/10.3390/agronomy9120848
Received: 27 August 2019 / Revised: 8 November 2019 / Accepted: 26 November 2019 / Published: 4 December 2019
(This article belongs to the Special Issue Potato Genetics and Breeding in the Genomics Era)
The potato is susceptible to water stress at all stages of development. We examined four clones of tetraploid potato, Cardinal, Desirée, Clone 37 FB, and Mije, from the germplasm bank of the National Institute of Agricultural Research (INIA) in Chile. Water stress was applied by suspending irrigation at the beginning of tuberization. Stomatal conductance, and tuber and plant fresh and dry weight were used to categorize water stress tolerance. Cardinal had a high susceptibility to water stress. Desirée was less susceptible than Cardinal and had some characteristics of tolerance. Mije had moderate tolerance and Clone 37 FB had high tolerance. Differential gene expression in leaves from plants with and without water stress were examined using transcriptome sequencing. Water stress-susceptible Cardinal had the fewest differentially expressed genes at 101, compared to Desirée at 1867, Clone 37 FB at 1179, and Mije at 1010. Water stress tolerance was associated with upregulation of the expression of transcription factor genes and genes involved in osmolyte and polyamine biosynthesis. Increased expression of genes encoding late embryogenesis abundant (LEA) and dehydrin proteins along with decreased expression of genes involved in nitrate assimilation and amino acid metabolism were found for clones showing water stress tolerance. The results also show that a water deficit was associated with reduced biotic stress responses. Additionally, heat shock protein genes were differentially expressed in all clones except for highly susceptible Cardinal. Together, the gene expression study demonstrates variation in the molecular pathways and biological processes in response to water stress contributing to tolerance and susceptibility.
Keywords: water stress; potato; RNAseq; tolerance. water stress; potato; RNAseq; tolerance.
MDPI and ACS Style

Barra, M.; Meneses, C.; Riquelme, S.; Pinto, M.; Lagüe, M.; Davidson, C.; Tai, H.H. Transcriptome Profiles of Contrasting Potato (Solanum tuberosum L.) Genotypes Under Water Stress. Agronomy 2019, 9, 848.

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