Genome-Wide Identification and Characterization of BPC Transcription Factors in Tobacco (Nicotiana tabacum)

Zhang, Ying; Jia, Shengwu; Wang, Sisi; Ji, Xiaolian; Liu, Zijin

doi:10.3390/agronomy15092084

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Genome-Wide Identification and Characterization of BPC Transcription Factors in Tobacco (Nicotiana tabacum)

by

Ying Zhang

^1,*,†,

Shengwu Jia

^2,†,

Sisi Wang

¹,

Xiaolian Ji

¹ and

Zijin Liu

^2,*

¹

College of Ecological and Environmental Engineering, Shaanxi A&F Technology University, Yangling 712100, China

²

Shaanxi Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, Yangling 712100, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Agronomy 2025, 15(9), 2084; https://doi.org/10.3390/agronomy15092084

Submission received: 30 July 2025 / Revised: 26 August 2025 / Accepted: 27 August 2025 / Published: 29 August 2025

(This article belongs to the Section Crop Breeding and Genetics)

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Abstract

BASIC PENTACYSTEINE (BPC) is a small family of plant-specific transcription factors that play crucial roles in plant growth, development processes, and response to abiotic stresses. However, the specific roles of Nicotiana tabacum BPCs (NtBPCs) remain ambiguous. Here, we identified 12 NtBPC genes, 5 of which were mapped to four chromosomes. Phylogenetic analysis classified these genes into three subfamilies. Collinearity was observed among BPC genes of N. tabacum, Capsicum annuum, and Solanum lycopersicum. Moreover, polypeptides encoded by NtBPC genes within the same subfamily shared similar conserved motifs and protein domains. Subcellular localization showed that 10 NtBPC proteins are localized in the nucleus. Promoter analysis revealed the presence of abiotic stress response elements in the promoters of NtBPCs. Further tissue-specific expression analysis using RT-qPCR revealed that NtBPCs are highly expressed in stems and leaves. After drought, NaCl, and cold treatments, NtBPCs exhibited varied expression patterns. These findings provide valuable insights into the evolutionary dynamics of the NtBPC gene family and lay the groundwork for subsequent investigations into the functions of NtBPC genes.

Keywords: BPC; expression pattern; genome-wide identification; Nicotiana tabacum

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MDPI and ACS Style

Zhang, Y.; Jia, S.; Wang, S.; Ji, X.; Liu, Z. Genome-Wide Identification and Characterization of BPC Transcription Factors in Tobacco (Nicotiana tabacum). Agronomy 2025, 15, 2084. https://doi.org/10.3390/agronomy15092084

AMA Style

Zhang Y, Jia S, Wang S, Ji X, Liu Z. Genome-Wide Identification and Characterization of BPC Transcription Factors in Tobacco (Nicotiana tabacum). Agronomy. 2025; 15(9):2084. https://doi.org/10.3390/agronomy15092084

Chicago/Turabian Style

Zhang, Ying, Shengwu Jia, Sisi Wang, Xiaolian Ji, and Zijin Liu. 2025. "Genome-Wide Identification and Characterization of BPC Transcription Factors in Tobacco (Nicotiana tabacum)" Agronomy 15, no. 9: 2084. https://doi.org/10.3390/agronomy15092084

APA Style

Zhang, Y., Jia, S., Wang, S., Ji, X., & Liu, Z. (2025). Genome-Wide Identification and Characterization of BPC Transcription Factors in Tobacco (Nicotiana tabacum). Agronomy, 15(9), 2084. https://doi.org/10.3390/agronomy15092084

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Genome-Wide Identification and Characterization of BPC Transcription Factors in Tobacco (Nicotiana tabacum)

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