Identification of Novel QTL for Seedling Root Architectural Traits in the D Genome of Natural and Resynthetic Allohexaploid Wheat
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Root System Architecture Phenotyping
2.3. Field Evaluation of Yield-Related Traits
2.4. QTL Analysis
2.5. InDel Markers Development
2.6. Prediction of Candidate Genes
2.7. Auxin Treatment
2.8. Detection of Candidate Gene Expression
2.9. Statistical Analysis
3. Results
3.1. Phenotypic Evaluation of Seminal Root Traits
3.2. Correlation among Seminal Root Traits and Yield Components
3.3. QTL Analysis
3.4. Fine Mapping and Verification of QLrn.qau-5D.2
3.5. Analysis of Candidate Genes
4. Discussion
4.1. Novel QTLs for Root-Related Traits in the D Genome of Wheat
4.2. Candidate Genes of QLrn.qau-5D.2
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Traits | Parents | RIL Population | HB2 (%) | ||||||
---|---|---|---|---|---|---|---|---|---|
TAA10 | XX329 | Difference | Max | Min | Average | SD | CV (%) | ||
LRN | 6.62 | 8.86 | −2.24 ** | 36.96 | 2.35 | 8.84 | 4.63 | 52.37 | 98.01 |
RHL | 1.16 | 0.81 | 0.35 *** | 1.41 | 0.53 | 0.96 | 0.17 | 17.75 | 98.49 |
SRN | 4.90 | 5.40 | −0.50 ** | 6.00 | 4.22 | 5.18 | 0.33 | 6.37 | 91.39 |
RD | 0.16 | 0.20 | −0.04 * | 0.20 | 0.13 | 0.17 | 0.07 | 41.18 | 76.25 |
TRV | 0.05 | 0.06 | −0.01 * | 0.14 | 0.02 | 0.05 | 0.01 | 27.92 | 84.48 |
Traits | SL | FSN | SSN | TSN | SC | PHT | HD | GL | GW | TGW |
---|---|---|---|---|---|---|---|---|---|---|
LRN | 0.022 | −0.077 | −0.003 | −0.076 | −0.077 | 0.145 | 0.151 * | 0.044 | −0.018 | −0.031 |
RHL | −0.034 | −0.047 | −0.042 | −0.063 | −0.004 | −0.083 | −0.144 | −0.003 | −0.023 | 0.043 |
SRN | 0.029 | 0.251 ** | −0.075 | 0.214 ** | 0.077 | −0.081 | −0.011 | 0.126 | 0.126 | 0.046 |
RD | −0.066 | −0.028 | 0.003 | −0.026 | 0.077 | −0.015 | 0.028 | 0.148 * | 0.209 ** | 0.306 ** |
RV | 0.015 | 0.014 | 0.082 | 0.046 | 0.011 | 0.101 | −0.003 | 0.168 * | 0.211 ** | 0.203 ** |
Trait | QTL | Nearest Marker | Position (cM) | LOD | Additive Value | CI (cM) | R2 (%) | Positive Allele |
---|---|---|---|---|---|---|---|---|
LRN | QLrn.qau-5D.1 | gwm190 | 3.95 | 2.55 | −1.06 | 1.4–10.2 | 4.98 | TAA10 |
QLrn.qau-5D.2 | 5D432 | 73.80 | 5.78 | −1.64 | 65.7–82.6 | 12.17 | TAA10 | |
QLrn.qau-7D | cfd68 | 92.29 | 2.84 | 1.11 | 90.9–97.1 | 5.35 | XX329 | |
SRN | QSrn.qau-2D | SSR2433 | 16.11 | 7.30 | 0.13 | 14.7–16.9 | 14.73 | XX329 |
RHL | QRhl.qau-4D | 4D237 | 16.60 | 4.24 | −0.06 | 15.5–17.3 | 9.32 | TAA10 |
RD | QRd.qau-3D | 2S250 | 56.20 | 2.86 | 0.0045 | 53.8–57.4 | 7.16 | XX329 |
TRV | QTrv.qau-5D | cfd183 | 121.90 | 2.95 | 0.0046 | 118.5–125.1 | 6.97 | XX329 |
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Wang, H.; Yang, B.; Zhao, X.; Chen, H.; Liu, F.; Ru, Y.; Wei, X.; Fu, X.; Guo, W.; Li, X.; et al. Identification of Novel QTL for Seedling Root Architectural Traits in the D Genome of Natural and Resynthetic Allohexaploid Wheat. Agronomy 2024, 14, 608. https://doi.org/10.3390/agronomy14030608
Wang H, Yang B, Zhao X, Chen H, Liu F, Ru Y, Wei X, Fu X, Guo W, Li X, et al. Identification of Novel QTL for Seedling Root Architectural Traits in the D Genome of Natural and Resynthetic Allohexaploid Wheat. Agronomy. 2024; 14(3):608. https://doi.org/10.3390/agronomy14030608
Chicago/Turabian StyleWang, Huifang, Bangbang Yang, Xinyu Zhao, Hailong Chen, Fei Liu, Yating Ru, Xirui Wei, Xiaofeng Fu, Weiwei Guo, Ximei Li, and et al. 2024. "Identification of Novel QTL for Seedling Root Architectural Traits in the D Genome of Natural and Resynthetic Allohexaploid Wheat" Agronomy 14, no. 3: 608. https://doi.org/10.3390/agronomy14030608