Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment

Park, Sol Lee; Cho, Jang Yeon; Kim, Su Hyun; Bhatia, Shashi Kant; Gurav, Ranjit; Park, See-Hyoung; Park, Kyungmoon; Yang, Yung-Hun

doi:10.3390/polym13234257

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Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment

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Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Korea

Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul 05029, Korea

Department of Biological and Chemical Engineering, Hongik University, Sejong 30016, Korea

Author to whom correspondence should be addressed.

Academic Editors: Rossella Dorati, Enrica Chiesa and Silvia Pisani

Polymers 2021, 13(23), 4257; https://doi.org/10.3390/polym13234257

Received: 2 November 2021 / Revised: 29 November 2021 / Accepted: 30 November 2021 / Published: 4 December 2021

(This article belongs to the Special Issue Degradation and Biological Application of Polymers)

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Abstract

Having the advantage of eco-friendly decomposition, bioplastics could be used to replace petroleum-based plastics. In particular, poly(3-hydroxybutyrate) (PHB) is one of the most commercialized bioplastics, however, necessitating the introduction of PHB-degrading bacteria for its effective disposal. In this study, Microbulbifer sp. SOL66 (94.18% 16S rRNA with similarity to Microbulbifer hydrolyticus) demonstrated the highest degradation activity among five newly screened Microbulbifer genus strains. Microbulbifer sp. SOL66 showed a rapid degradation yield, reaching 98% in 4 days, as monitored by laboratory scale, gas chromatography-mass spectrometry, scanning electron microscopy, gel permeation chromatography, and Fourier transform infrared spectroscopy. The PHB film was completely degraded within 7 days at 37 °C in the presence of 3% NaCl. When 1% xylose and 0.4% ammonium sulfate were added, the degradation activity increased by 17% and 24%, respectively. In addition, this strain showed biodegradability on pellets of poly(3-hydroxybutyrate-co-4-hydroxybutyrate), as confirmed by weight loss and physical property changes. We confirmed that Microbulbifer sp. SOL66 has a great ability to degrade PHB, and has rarely been reported to date. View Full-Text

Keywords: poly(3-hydroxybutyrate); bioplastics; biodegradation; screening; Microbulbifer genus

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MDPI and ACS Style

Park, S.L.; Cho, J.Y.; Kim, S.H.; Bhatia, S.K.; Gurav, R.; Park, S.-H.; Park, K.; Yang, Y.-H. Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment. Polymers 2021, 13, 4257. https://doi.org/10.3390/polym13234257

AMA Style

Park SL, Cho JY, Kim SH, Bhatia SK, Gurav R, Park S-H, Park K, Yang Y-H. Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment. Polymers. 2021; 13(23):4257. https://doi.org/10.3390/polym13234257

Chicago/Turabian Style

Park, Sol L., Jang Y. Cho, Su H. Kim, Shashi K. Bhatia, Ranjit Gurav, See-Hyoung Park, Kyungmoon Park, and Yung-Hun Yang. 2021. "Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment" Polymers 13, no. 23: 4257. https://doi.org/10.3390/polym13234257

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Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment

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