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Article

Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems

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Associate Laboratory i4HB—Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal
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UCIBIO—Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal
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CENIMAT/I3N, Department of Physics, NOVA School of Science and Technology, Universidade Nova de Lisboa, 2819-516 Caparica, Portugal
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LAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, 2819-516 Caparica, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Jean Duhamel
Polymers 2021, 13(18), 3020; https://doi.org/10.3390/polym13183020
Received: 30 July 2021 / Revised: 2 September 2021 / Accepted: 3 September 2021 / Published: 7 September 2021
(This article belongs to the Special Issue Polymeric Biomaterials and Drug Delivery Systems)
Reactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by exogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by Enterobacter A47, containing ca. 17 wt% of negatively charged residues in its structure. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe3+-TPTZ species at an EC50 of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% w/v FucoPol concentration shown previously to be cryo- and photoprotective was also demonstrated to protect Vero cells against H2O2-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality. View Full-Text
Keywords: FucoPol; polysaccharide; antioxidant; stress; FRAP; Hill; hydrogen peroxide; food; radiation; cryopreservation FucoPol; polysaccharide; antioxidant; stress; FRAP; Hill; hydrogen peroxide; food; radiation; cryopreservation
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MDPI and ACS Style

Guerreiro, B.M.; Silva, J.C.; Lima, J.C.; Reis, M.A.M.; Freitas, F. Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems. Polymers 2021, 13, 3020. https://doi.org/10.3390/polym13183020

AMA Style

Guerreiro BM, Silva JC, Lima JC, Reis MAM, Freitas F. Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems. Polymers. 2021; 13(18):3020. https://doi.org/10.3390/polym13183020

Chicago/Turabian Style

Guerreiro, Bruno M., Jorge C. Silva, João C. Lima, Maria A.M. Reis, and Filomena Freitas. 2021. "Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems" Polymers 13, no. 18: 3020. https://doi.org/10.3390/polym13183020

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