Next Article in Journal
Graphene Oxide Composite for Selective Recognition, Capturing, Photothermal Killing of Bacteria over Mammalian Cells
Previous Article in Journal
The Use of Poly(N-vinyl pyrrolidone) in the Delivery of Drugs: A Review
Previous Article in Special Issue
Functional Micro- and Nanofibers Obtained by Nonwoven Post-Modification
Open AccessArticle

Anti-Escherichia coli Functionalized Silver-Doped Carbon Nanofibers for Capture of E. coli in Microfluidic Systems

1
Department of Fiber Science and Apparel Design, Cornell University, Ithaca, NY 14853, USA
2
Robert Frederick Smith School of Chemical & Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA
*
Author to whom correspondence should be addressed.
Polymers 2020, 12(5), 1117; https://doi.org/10.3390/polym12051117
Received: 29 March 2020 / Revised: 27 April 2020 / Accepted: 11 May 2020 / Published: 13 May 2020
(This article belongs to the Special Issue Functional Electrospun Nanofibers)
Silver-doped carbon nanofibers (SDCNF) are used as the base material for the selective capture of Escherichia coli in microfluidic systems. Fibers were spun in a glovebox with dry atmosphere maintained by forced dry air pumped through the closed environment. This affected the evaporation rate of the solvent during the electrospinning process and the distribution of silver particles within the fiber. Antibodies are immobilized on the surface of the silver-doped polyacrylonitrile (PAN) based carbon nanofibers via a three-step process. The negatively charged silver particles present on the surface of the nanofibers provide suitable sites for positively charged biotinylated poly-(L)-lysine-graft-poly-ethylene-glycol (PLL-g-PEG biotin) conjugate attachment. Streptavidin and a biotinylated anti-E. coli antibody were then added to create anti-E. coli surface functionalized (AESF) nanofibers. Functionalized fibers were able to immobilize up to 130 times the amount of E. coli on the fiber surface compared to neat silver doped fibers. Confocal images show E. coli remains immobilized on fiber mat surface after extensive rinsing showing the bacteria is not simply a result of non-specific binding. To demonstrate selectivity and functionalization with both gram negative and gram-positive antibodies, anti-Staphylococcus aureus surface functionalized (ASSF) nanofibers were also prepared. Experiments with AESF performed with Staphylococcus aureus (S. aureus) and ASSF with E. coli show negligible binding to the fiber surface showing the selectivity of the functionalized membranes. This surface functionalization can be done with a variety of antibodies for tunable selective pathogen capture. View Full-Text
Keywords: functional materials via electrospinning method; microfluidics; Escherichia coli; Staphylococcus aureus; silver doped; selective capture functional materials via electrospinning method; microfluidics; Escherichia coli; Staphylococcus aureus; silver doped; selective capture
Show Figures

Graphical abstract

MDPI and ACS Style

Smith, S.; Delaney, M.; Frey, M. Anti-Escherichia coli Functionalized Silver-Doped Carbon Nanofibers for Capture of E. coli in Microfluidic Systems. Polymers 2020, 12, 1117.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop