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Open AccessArticle

Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

by Yannan Liu 1,2,3,†, Juan Gu 1,2,3,† and Daidi Fan 1,2,3,*
1
Shanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi′an 710069, China
2
Shanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi′an 710069, China
3
Biotech. & Biomed. Research Institute, Northwest University, Xi’an 710069, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Polymers 2020, 12(1), 61; https://doi.org/10.3390/polym12010061
Received: 24 November 2019 / Revised: 11 December 2019 / Accepted: 17 December 2019 / Published: 1 January 2020
A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects. View Full-Text
Keywords: scaffolds; bone tissue regeneration; biocompatibility; nano-hydroxyapatite; human-like collagen scaffolds; bone tissue regeneration; biocompatibility; nano-hydroxyapatite; human-like collagen
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MDPI and ACS Style

Liu, Y.; Gu, J.; Fan, D. Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration. Polymers 2020, 12, 61.

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