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Open AccessArticle

Effects of Different Temperatures on the Chemical Structure and Antitumor Activities of Polysaccharides from Cordyceps militaris

1
College of Life Sciences, Northeast Normal University, Changchun 130024, China
2
College of Biological and Geographic Sciences, Yili Normal University, Yining 835000, China
3
Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
*
Authors to whom correspondence should be addressed.
Polymers 2018, 10(4), 430; https://doi.org/10.3390/polym10040430
Received: 12 March 2018 / Revised: 25 March 2018 / Accepted: 9 April 2018 / Published: 12 April 2018
(This article belongs to the Collection Polysaccharides)
The effects of different extraction temperatures (4 and 80 °C) on the physicochemical properties and antitumor activity of water soluble polysaccharides (CMPs-4 and CMPs-80) from Cordyceps militaris (C. militaris) were evaluated in this study. The results of gas chromatography (GC) and high-performance gel permeation chromatography (HPGPC) showed that a higher extraction temperature could degrade the polysaccharides with 188 kDa, mainly composed of glucose, and increase the dissolution rate of polysaccharides about 308 kDa, mainly consisting of rhamnose and galactose. In addition, the CMPs displayed the same sugar ring and category of glycosidic linkage based on Fourier-transform infrared spectroscopy (FTIR) analysis, however, their invisible structural difference occurred in the specific rotation and conformational characteristics according to the results of specific optical rotation measurement and Congo red test. In vitro antitumor experiments indicated that CMPs-4 possessed stronger inhibitory effects on human esophagus cancer Eca-109 cells by inducing cell apoptosis more than CMPs-80 did. These findings demonstrated that the polysaccharides extracted with cold water (4 °C) could be applied as a novel alternative chemotherapeutic agent or dietary supplement with its underlying antitumor property. View Full-Text
Keywords: C. militaris; extraction temperature; polysaccharides; physicochemical properties; antitumor activity C. militaris; extraction temperature; polysaccharides; physicochemical properties; antitumor activity
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MDPI and ACS Style

Nurmamat, E.; Xiao, H.; Zhang, Y.; Jiao, Z. Effects of Different Temperatures on the Chemical Structure and Antitumor Activities of Polysaccharides from Cordyceps militaris. Polymers 2018, 10, 430.

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