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Polymers 2018, 10(11), 1275; https://doi.org/10.3390/polym10111275

Fabrication of Carbohydrate Chips Based on Polydopamine for Real-Time Determination of Carbohydrate–Lectin Interactions by QCM Biosensor

1
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China
2
Attana AB, SE-11419 Stockholm, Sweden
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 17 October 2018 / Revised: 8 November 2018 / Accepted: 12 November 2018 / Published: 16 November 2018
(This article belongs to the Special Issue Polymer Based Bio-Sensors)
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Abstract

A novel approach for preparing carbohydrate chips based on polydopamine (PDA) surface to study carbohydrate–lectin interactions by quartz crystal microbalance (QCM) biosensor instrument has been developed. The amino-carbohydrates were immobilized on PDA-coated quartz crystals via Schiff base reaction and/or Michael addition reaction. The resulting carbohydrate-chips were applied to QCM biosensor instrument with flow-through system for real-time detection of lectin–carbohydrate interactions. A series of plant lectins, including wheat germ agglutinin (WGA), concanavalin A (Con A), Ulex europaeus agglutinin I (UEA-I), soybean agglutinin (SBA), and peanut agglutinin (PNA), were evaluated for the binding to different kinds of carbohydrate chips. Clearly, the results show that the predicted lectin selectively binds to the carbohydrates, which demonstrates the applicability of the approach. Furthermore, the kinetics of the interactions between Con A and mannose, WGA and N-Acetylglucosamine were studied, respectively. This study provides an efficient approach to preparing carbohydrate chips based on PDA for the lectin–carbohydrate interactions study. View Full-Text
Keywords: polydopamine; amino-carbohydrates; carbohydrate chips; QCM biosensor; carbohydrate–lectin interactions polydopamine; amino-carbohydrates; carbohydrate chips; QCM biosensor; carbohydrate–lectin interactions
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Shang, K.; Song, S.; Cheng, Y.; Guo, L.; Pei, Y.; Lv, X.; Aastrup, T.; Pei, Z. Fabrication of Carbohydrate Chips Based on Polydopamine for Real-Time Determination of Carbohydrate–Lectin Interactions by QCM Biosensor. Polymers 2018, 10, 1275.

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