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Catalysts 2019, 9(2), 206; https://doi.org/10.3390/catal9020206

Isolation of a Bacillus Aryabhattai Strain for the Resolution of (R, S)-Ethyl Indoline-2-Carboxylate to Produce (S)-Indoline-2-Carboxylic Acid

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China
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Received: 27 January 2019 / Revised: 18 February 2019 / Accepted: 20 February 2019 / Published: 25 February 2019
(This article belongs to the Special Issue Biocatalysts: Design and Application)
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Abstract

The strain screened from sludge can selectively hydrolyze (S)-ethyl indoline-2-carboxylate to produce (S)-indoline-2-carboxylic acid. It was identified as the Bacillus aryabhattai strain based on its morphology, metabolism, and 16S rDNA sequence analysis. Glucose and yeast powder were used as the best carbon and nitrogen sources to cultured cells with an initial pH of seven. Subsequently, we optimized the key parameters for selective hydrolysis. Finally, when the substrate concentration had reached 3%, with a 35 °C water bath, a pH of seven, and a speed of 600 rpm, the e.e.p value attained 96% with a 33% yield. Thus, we had developed a new method for producing (S)-indoline-2-carboxylic acid that used whole microbial cells as the biocatalyst. View Full-Text
Keywords: (S)-indoline-2-carboxylic acid; whole cell catalysis; esterase; perindopril (S)-indoline-2-carboxylic acid; whole cell catalysis; esterase; perindopril
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Zhang, Y.; Chen, J.; Chen, C.; Wu, S. Isolation of a Bacillus Aryabhattai Strain for the Resolution of (R, S)-Ethyl Indoline-2-Carboxylate to Produce (S)-Indoline-2-Carboxylic Acid. Catalysts 2019, 9, 206.

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