Next Article in Journal
Enhancing the Water Resistance of Mn-MOF-74 by Modification in Low Temperature NH3-SCR
Previous Article in Journal
Energy-Saving UHMW Polymeric Flow Aids: Catalyst and Polymerization Process Development
Open AccessArticle

Enhanced Lycopene Production in Escherichia coli by Expression of Two MEP Pathway Enzymes from Vibrio sp. Dhg

1
Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea
2
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Catalysts 2019, 9(12), 1003; https://doi.org/10.3390/catal9121003
Received: 28 October 2019 / Revised: 21 November 2019 / Accepted: 26 November 2019 / Published: 29 November 2019
(This article belongs to the Section Biocatalysis)
Microbial production is a promising method that can overcome major limitations in conventional methods of lycopene production, such as low yields and variations in product quality. Significant efforts have been made to improve lycopene production by engineering either the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway or mevalonate (MVA) pathway in microorganisms. To further improve lycopene production, it is critical to utilize metabolic enzymes with high specific activities. Two enzymes, 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and farnesyl diphosphate synthase (IspA), are required in lycopene production using MEP pathway. Here, we evaluated the activities of Dxs and IspA of Vibrio sp. dhg, a newly isolated and fast-growing microorganism. Considering that the MEP pathway is closely related to the cell membrane and electron transport chain, the activities of the two enzymes of Vibrio sp. dhg were expected to be higher than the enzymes of Escherichia coli. We found that Dxs and IspA in Vibrio sp. dhg exhibited 1.08-fold and 1.38-fold higher catalytic efficiencies, respectively. Consequently, the heterologous overexpression improved the specific lycopene production by 1.88-fold. Our findings could be widely utilized to enhance production of lycopene and other carotenoids. View Full-Text
Keywords: metabolic engineering; lycopene; MEP pathway; 1-deoxy-d-xylulose-5-phosphate synthase; farnesyl diphosphate synthase; Vibrio sp. dhg metabolic engineering; lycopene; MEP pathway; 1-deoxy-d-xylulose-5-phosphate synthase; farnesyl diphosphate synthase; Vibrio sp. dhg
Show Figures

Figure 1

MDPI and ACS Style

Kim, M.J.; Noh, M.H.; Woo, S.; Lim, H.G.; Jung, G.Y. Enhanced Lycopene Production in Escherichia coli by Expression of Two MEP Pathway Enzymes from Vibrio sp. Dhg. Catalysts 2019, 9, 1003.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop