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Open AccessArticle

Screening and Comparative Characterization of Microorganisms from Iranian Soil Samples Showing ω-Transaminase Activity toward a Plethora of Substrates

1
Department of Biotechnology, Faculty of Biology Science, Alzahra University, 1993893973 Tehran, Iran
2
BLT_II: Technical Biology, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 4, 76131 Karlsruhe, Germany
3
Department of Chemistry, School of Sciences, Alzahra University, 1993891176 Tehran, Iran
4
Higher Education Complex of Shirvan, 9468194477 Shirvan, Iran
*
Authors to whom correspondence should be addressed.
Catalysts 2019, 9(10), 874; https://doi.org/10.3390/catal9100874
Received: 26 September 2019 / Revised: 15 October 2019 / Accepted: 18 October 2019 / Published: 22 October 2019
(This article belongs to the Special Issue Novel Enzyme and Whole-Cell Biocatalysts)
In this study, soil microorganisms from Iran were screened for ω-transaminase (ω-TA) activity based on growth on minimal media containing (rac)-α-methylbenzylamine (rac-α-MBA) as a sole nitrogen source. Then, for the selection of strains with high enzyme activity, a colorimetric o-xylylendiamine assay was conducted. The most promising strains were identified by 16S rDNA sequencing. Five microorganisms showing high ω-TA activity were subjected to determine optimal conditions for ω-TA activity, including pH, temperature, co-solvent, and the specificity of the ω-TA toward different amine donors and acceptors. Among the five screened microorganisms, Bacillus halotolerans turned out to be the most promising strain: Its cell-free extract showed a highly versatile amino donor spectrum toward aliphatic, aromatic chiral amines and a broad range of pH activity. Transaminase activity also exhibited excellent solvent tolerance, with maximum turnover in the presence of 30% (v/v) DMSO. View Full-Text
Keywords: ω-transaminase; α-methylbenzylamine; biocatalysis; chiral amine; biotransformation ω-transaminase; α-methylbenzylamine; biocatalysis; chiral amine; biotransformation
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MDPI and ACS Style

Gord Noshahri, N.; Fooladi, J.; Syldatk, C.; Engel, U.; Heravi, M.M.; Zare Mehrjerdi, M.; Rudat, J. Screening and Comparative Characterization of Microorganisms from Iranian Soil Samples Showing ω-Transaminase Activity toward a Plethora of Substrates. Catalysts 2019, 9, 874.

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