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Open AccessArticle

Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by Entrapment in Polyvinyl Alcohol and Its Application to Neo-Fructooligosaccharides Production

1
Instituto de Catálisis y Petroleoquímica, CSIC, 28049 Madrid, Spain
2
Centro de Biología Molecular Severo Ochoa, CSIC-UAM, 28049 Madrid, Spain
3
Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, 1649-003 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Catalysts 2018, 8(5), 201; https://doi.org/10.3390/catal8050201
Received: 21 April 2018 / Revised: 7 May 2018 / Accepted: 9 May 2018 / Published: 11 May 2018
(This article belongs to the Special Issue Immobilized Biocatalysts)
The β-fructofuranosidase (Xd-INV) from the basidiomycota yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) is unique in its ability to synthesize neo- fructooligosaccharides (neo-FOS). In order to facilitate its industrial application, the recombinant enzyme expressed in Pichia pastoris (pXd-INV) was immobilized by entrapment in polyvinyl alcohol (PVA) hydrogels. The encapsulation efficiency exceeded 80%. The PVA lenticular particles of immobilized pXd-INV were stable up to approximately 40 °C. Using 600 g/L sucrose, the immobilized biocatalyst synthesized 18.9% (w/w) FOS (59.1 g/L of neokestose, 30.2 g/L of 1-kestose, 11.6 g/L of neonystose and 12.6 g/L of blastose). The operational stability of PVA-immobilized biocatalyst was assayed in a batch reactor at 30 °C. The enzyme preserved its initial activity during at least 7 cycles of 26 h. View Full-Text
Keywords: glycosidases; fructooligosaccharides; prebiotics; enzyme entrapment; immobilization; bioreactors; hydrogels; neokestose glycosidases; fructooligosaccharides; prebiotics; enzyme entrapment; immobilization; bioreactors; hydrogels; neokestose
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Míguez, N.; Gimeno-Pérez, M.; Fernández-Polo, D.; Cervantes, F.V.; Ballesteros, A.O.; Fernández-Lobato, M.; Ribeiro, M.H.; Plou, F.J. Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by Entrapment in Polyvinyl Alcohol and Its Application to Neo-Fructooligosaccharides Production. Catalysts 2018, 8, 201.

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