Next Article in Journal
A Robust Two-Step Process for the Efficient Conversion of Acidic Soybean Oil for Biodiesel Production
Next Article in Special Issue
Infrared Characterization of the Bidirectional Oxygen-Sensitive [NiFe]-Hydrogenase from E. coli
Previous Article in Journal
Prediction of In-Situ Gasification Chemical Looping Combustion Effects of Operating Conditions
Previous Article in Special Issue
Immobilization of Eversa Lipase on Octyl Agarose Beads and Preliminary Characterization of Stability and Activity Features
Open AccessFeature PaperArticle

Efficient Conversion of Acetate to 3-Hydroxypropionic Acid by Engineered Escherichia coli

1
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea
2
Department of Chemical Engineering and, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Catalysts 2018, 8(11), 525; https://doi.org/10.3390/catal8110525
Received: 17 October 2018 / Revised: 1 November 2018 / Accepted: 3 November 2018 / Published: 7 November 2018
Acetate, which is an abundant carbon source, is a potential feedstock for microbial processes that produce diverse value-added chemicals. In this study, we produced 3-hydroxypropionic acid (3-HP) from acetate with engineered Escherichia coli. For the efficient conversion of acetate to 3-HP, we initially introduced heterologous mcr (encoding malonyl-CoA reductase) from Chloroflexus aurantiacus. Then, the acetate assimilating pathway and glyoxylate shunt pathway were activated by overexpressing acs (encoding acetyl-CoA synthetase) and deleting iclR (encoding the glyoxylate shunt pathway repressor). Because a key precursor malonyl-CoA is also consumed for fatty acid synthesis, we decreased carbon flux to fatty acid synthesis by adding cerulenin. Subsequently, we found that inhibiting fatty acid synthesis dramatically improved 3-HP production (3.00 g/L of 3-HP from 8.98 g/L of acetate). The results indicated that acetate can be used as a promising carbon source for microbial processes and that 3-HP can be produced from acetate with a high yield (44.6% of the theoretical maximum yield). View Full-Text
Keywords: metabolic engineering; synthetic biology; 3-hydroxypropionic acid; microbial production; fatty acid synthesis; acetate metabolic engineering; synthetic biology; 3-hydroxypropionic acid; microbial production; fatty acid synthesis; acetate
Show Figures

Graphical abstract

MDPI and ACS Style

Lee, J.H.; Cha, S.; Kang, C.W.; Lee, G.M.; Lim, H.G.; Jung, G.Y. Efficient Conversion of Acetate to 3-Hydroxypropionic Acid by Engineered Escherichia coli. Catalysts 2018, 8, 525.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop