Special Issue "Recent Advances in Biocatalysis and Metabolic Engineering for Biomanufacturing"
Deadline for manuscript submissions: 31 March 2019
The use of biocatalysts, including enzyme and metabolically engineered cells, has attracted a great deal of attention in chemical and bioindustry because biocatalytic reactions can be conducted under environmentally-benign conditions and in sustainable ways. The catalytic efficiency and chemo-, regio-, stereoselectivity of enzyme can be readily enhanced and modulated using protein engineering. Metabolic engineering seeks to enhance cellular biosynthetic productivity of target metabolites via controlling and redesigning metabolic pathways using multi-omics analysis, genome-scale modeling, metabolic flux control and reconstruction of novel pathways.
The aim of this Special Issue is to deal with the recent advances in biocatalysis and metabolic engineering for biomanufacturing of fuels, chemicals, biomaterials, and pharmaceuticals. Reviews and original research papers on the development of new strategies to improve the catalytic efficiency of enzyme, biosynthetic capability of cell factory and their applications in production of various bioproducts and chemicals are welcome.
Prof. Eun Yeol Lee
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Catalysts is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- Biocatalytic reaction
- Protein Engineering
- Metabolic Engineering
- Molecular Biotechnology
- Cell factory
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Exploring metagenomic enzymes: a novel esterase useful for short-chain ester synthesis
Authors: Thaís Carvalho Maester1,2,a, Mariana Rangel Pereira1,2,b, Aliandra Maura Gibertoni Malaman1; Janaina Pires Borges3; Eleni Gomes4; Eliana Gertrudes de Macedo Lemos1*
Affiliation: 1Department of Technology, São Paulo State University (UNESP), Jaboticabal, SP, Brazil;
2University of São Paulo (USP), São Paulo, SP, Brazil;
3Institute of Biosciences, Languages and Exact Sciences, Department of Chemistry and Environmental Sciences, São Paulo State University (UNESP), São José do Rio Preto, SP, Brazil;
4Institute of Biosciences, Languages and Exact Sciences, Department of Biology, São Paulo State University (UNESP), São José do Rio Preto, SP, Brazil;
Present address: aSupera Innovation and Technology Park, Ecobiotech company, Ribeirão Preto, SP, Brazil;
b Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom;
Corresponding author: Eliana G. M. Lemos: [email protected]
Abstract： Lipolytic enzymes have gained attention due to their remarkable ability to carry out a variety of reactions. Enzyme-mediated esterification reactions can be a promising alternative to produce esters of commercial interest, replacing conventional chemical processes. In this study, the novel esterase-encoding gene est5 from a metagenomic library isolated from a microbial consortium specialized in diesel oil degradation was cloned and expressed in Escherichia coli. Sequence analysis revealed a 912 bp ORF encoding a 340-amino acid polypeptide with predicted molecular weight of 37 kDa that belongs to family V of the lipolytic enzymes. Recombinant EST5 was purified and presented higher activity at pH 7.5, 45°C, with a Tm of 47°C, although remained at least 50% active at low temperatures. EST5 exhibited broad substrate specificity toward p-nitrophenol esters with highest activity for p-nitrophenyl valerate (Kcat/Km rate of 1533.27 s-1.mM-1) and remained stable in the presence of up to 10% methanol and 20% dimethyl sulfoxide. Preliminary studies of EST5 in the synthesis of methyl butyrate led to a specific activity of 127.04 U.mg-1. The enzyme showed higher esterification activity than other literature results, including commercial enzymes. EST5 has potential for synthesis of flavor esters, providing a concept for its application in biotechnological processes.
Keywords: Lipolytic enzymes; Metagenome; Family V; Esterification; Flavor esters