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20 December 2025

Endoscopic Tattooing Using Indocyanine Green (ICG) Fluorescence for Intraoperative Guidance in Colorectal Surgery: Review of the Literature

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1
Third Department of Surgery, Evaggelismos General Hospital of Athens, 10676 Athens, Greece
2
Department of Gastroenterology, General Hospital of Nea Ionia “Konstantopouleio-Patision”, 14233 Athens, Greece
3
Hepatogastroenterology Unit, Second Department of Internal Medicine-Propaedeutic, Attikon University Hospital, Rimini 1, Chaidari, 12462 Athens, Greece
4
Second Department of Surgery, “G.Gennimatas” General Hospital of Athens, 11527 Athens, Greece
Cancers2026, 18(1), 22;https://doi.org/10.3390/cancers18010022 
(registering DOI)
This article belongs to the Special Issue Novel Concepts in Gastrointestinal Surgical Oncology

Simple Summary

Indocyanine green (ICG) has been recently described in endoscopic tattooing of colorectal lesions treated with curative-intent surgery combined with near-infrared laparoscopic and robotic platforms. Our systematic review of the literature has focused on its utility in intraoperative tumor identification rates, and in identifying tumor-associated lymphatic drainage and performing radical lymphadenectomy. We have also described protocols used in timing and dosages used in ICG-based tattooing as well as associated adverse events. Significant potential benefits in all aforementioned aspects have been noted. However, existing data suffer from significant limitations.

Abstract

Background and Objectives: Accurate endoscopic lesion localization is crucial for planning and performing curative-intent surgery in colorectal cancer management. The use of indocyanine green (ICG) has been described as a novel alternative for colorectal lesion marking. Materials and Methods: We have performed a systematic review of the literature on the use of ICG-based tattooing for patients with colorectal cancer undergoing surgery. Results: A total of 19 studies were identified. Seven studies reposted the rates of successful intraoperative localization following ICG tattooing. Additionally, six studies provided detailed descriptions of the administration protocols, including both timing and dosage. A total of twelve studies described the utility of ICG-based tattooing for subsequent lymphadenectomy and its oncologic implications. Lymphadenectomy under fluorescent guidance was associated with increased lymph node yields as well as a change to the surgical lymphadenectomy plan in a significant proportion of patients. Conclusions: ICG-based endoscopic tattooing has demonstrated significant value in facilitating precise lesion localization during curative-intent colorectal surgery. Moreover, its use has been extended to guiding lymphadenectomy, with reports indicating improved lymph node yields. Nevertheless, further research is required to standardize protocols and address existing limitations.

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