Febrile Neutropenia Duration Is Associated with the Severity of Gut Microbiota Dysbiosis in Pediatric Allogeneic Hematopoietic Stem Cell Transplantation Recipients
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Population and Stool Sampling
2.2. Microbial DNA Extraction and 16S rRNA Gene Library Preparation
2.3. Bioinformatic and Statistical Analysis
3. Results
3.1. Study Cohort Description
3.2. Gut Microbiota Dynamics in Pediatric Allo-HSCT Patients in Relation to the Duration of Febrile Neutropenia
3.3. Early and Late Gut Microbiota Signatures of Febrile Neutropenia Duration in Pediatric Allo-HSCT Patients
3.4. Association between Febrile Neutropenia Duration and Development of Other Transplant Complications
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristic | Overall (N = 37) | Bologna (N = 12) | Verona (N = 12) | Wroclaw (N = 13) |
---|---|---|---|---|
Age at HSCT—year | 8.3 (1.0–18.0) | 8.8 (1.1–18.0) | 9.0 (1.2–17.6) | 7.2 (1.0–13.7) |
Malignant disease—no. (%) | 22 (59) | 8 (67) | 8 (67) | 6 (46) |
Donor—no. (%) | ||||
MUD | 20 (54) | 6 (50) | 7 (58) | 7 (54) |
MMUD | 7 (19) | 2 (17) | 4 (33) | 1 (8) |
Haplo | 5 (14) | 3 (25) | 0 (19) | 2 (15) |
MSD | 5 (14) | 1 (8) | 1 (8) | 3 (23) |
Graft type—no. (%) | ||||
BM | 22 (60) | 9 (75) | 8 (67) | 5 (38) |
PBSC | 15 (40) | 3 (25) | 4 (33) | 8 (62) |
Intensity of conditioning regimen—no. (%) | ||||
MAC | 24 (65) | 10 (83) | 7 (58) | 7 (54) |
RIC | 13 (35) | 2 (17) | 5 (42) | 6 (46) |
Fever > 3 Days (n = 16) | Fever ≤ 3 Days (n = 21) | p | |
---|---|---|---|
Center: | 0.136 | ||
Bologna | 8 (50.0%) | 4 (19.0%) | |
Wroclaw | 4 (25.0%) | 9 (42.9%) | |
Verona | 4 (25.0%) | 8 (38.1%) | |
Underlying disease: | 0.742 | ||
Malignant | 10 (62.5%) | 12 (57.1%) | |
Non-malignant | 6 (37.5%) | 9 (42.9%) | |
Antibiotic prophylaxis: | 0.141 | ||
Yes | 6 (37.5%) | 13 (61.9%) | |
No | 10 (62.5%) | 8 (38.1%) | |
Type of conditioning: | 0.260 | ||
MAC | 12 (75.0%) | 12 (57.1%) | |
RIC | 4 (25.0%) | 9 (42.9%) | |
Use of granulocyte colony-stimulating factor (G-CSF): | 0.384 | ||
Yes | 14 (87.5%) | 16 (76.2%) | |
No | 2 (12.5%) | 5 (23.8%) | |
Corticosteroid for GvHD: | 0.208 | ||
Yes | 8 (50.0%) | 7 (33.3%) | |
No | 2 (12.5%) | 0 (0%) | |
Bloodstream infections: | 0.384 | ||
Yes | 2 (12.5%) | 5 (23.8%) | |
No | 14 (87.5%) | 16 (76.2%) | |
GvHD (any grade): | 0.104 | ||
Yes | 10 (62.5%) | 7 (33.3%) | |
No | 6 (37.5%) | 14 (66.7%) | |
GvHD (grade II-IV): | 0.705 | ||
Yes | 4 (25.0%) | 4 (19.0%) | |
No | 12 (75.0%) | 17 (81.0%) | |
Nutrition: | 1.00 | ||
EN | 6 (37.5%) | 8 (38.1%) | |
PN | 10 (62.5%%) | 13 (61.9%) | |
Mucositis: | 0.733 | ||
Grade 3–4 | 7 (43.8%) | 7 (33.3%) | |
Grade ≤ 2 | 9 (56.2%) | 14 (66.7%) | |
Graft source: | 0.176 | ||
BM | 12 (75.0%) | 10 (47.6%) | |
PBSC | 4 (25.0%) | 11 (52.4%) | |
Donor: | 0.460 | ||
MUD | 7 (43.8%) | 13 (61.9%) | |
MMUD | 4 (25.0%) | 4 (19.0%) | |
Haplo | 4 (25.0%) | 2 (9.5%) | |
MSD | 1 (6.2%) | 3 (14.3%) |
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Masetti, R.; D’Amico, F.; Zama, D.; Leardini, D.; Muratore, E.; Ussowicz, M.; Fraczkiewicz, J.; Cesaro, S.; Caddeo, G.; Pezzella, V.; et al. Febrile Neutropenia Duration Is Associated with the Severity of Gut Microbiota Dysbiosis in Pediatric Allogeneic Hematopoietic Stem Cell Transplantation Recipients. Cancers 2022, 14, 1932. https://doi.org/10.3390/cancers14081932
Masetti R, D’Amico F, Zama D, Leardini D, Muratore E, Ussowicz M, Fraczkiewicz J, Cesaro S, Caddeo G, Pezzella V, et al. Febrile Neutropenia Duration Is Associated with the Severity of Gut Microbiota Dysbiosis in Pediatric Allogeneic Hematopoietic Stem Cell Transplantation Recipients. Cancers. 2022; 14(8):1932. https://doi.org/10.3390/cancers14081932
Chicago/Turabian StyleMasetti, Riccardo, Federica D’Amico, Daniele Zama, Davide Leardini, Edoardo Muratore, Marek Ussowicz, Jowita Fraczkiewicz, Simone Cesaro, Giulia Caddeo, Vincenza Pezzella, and et al. 2022. "Febrile Neutropenia Duration Is Associated with the Severity of Gut Microbiota Dysbiosis in Pediatric Allogeneic Hematopoietic Stem Cell Transplantation Recipients" Cancers 14, no. 8: 1932. https://doi.org/10.3390/cancers14081932
APA StyleMasetti, R., D’Amico, F., Zama, D., Leardini, D., Muratore, E., Ussowicz, M., Fraczkiewicz, J., Cesaro, S., Caddeo, G., Pezzella, V., Belotti, T., Gottardi, F., Tartari, P., Brigidi, P., Turroni, S., & Prete, A. (2022). Febrile Neutropenia Duration Is Associated with the Severity of Gut Microbiota Dysbiosis in Pediatric Allogeneic Hematopoietic Stem Cell Transplantation Recipients. Cancers, 14(8), 1932. https://doi.org/10.3390/cancers14081932