The Long Noncoding RNA LOC441461 (STX17-AS1) Modulates Colorectal Cancer Cell Growth and Motility
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Expression Levels of LOC441461 Were Significantly Increased in CRC
2.2. LOC441461 Expressed with Cancer-Related Signaling Pathway Dysfunction
2.3. LOC441461 Regulated Colon Cancer Cell Growth by Impairing Cell Cycle Progression
2.4. LOC441461 Involved in Colon Cancer Motility
3. Discussion
4. Materials and Methods
4.1. Colon Cancer Cell Line
4.2. Clinical Samples
4.3. Ethics Approval and Consent to Participate
4.4. Extraction of RNA and Reverse Transcription
4.5. RT-PCR
4.6. Expression Data from TCGA
4.7. Pathway Enrichment Analysis
4.8. siRNA Transfection
4.9. Proliferation
4.10. Soft Agar Assay
4.11. Colony Formation Assay
4.12. Cell Cycle Analysis
4.13. Apoptotic Cells Stained with Annexin V, Propidium Iodide, and Hoechst 33342
4.14. Invasion and Migration Assay
4.15. Western Blotting
4.16. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, J.-H.; Lu, T.-J.; Kung, M.-L.; Yang, Y.-F.; Yeh, C.-Y.; Tu, Y.-T.; Chen, W.-S.; Tsai, K.-W. The Long Noncoding RNA LOC441461 (STX17-AS1) Modulates Colorectal Cancer Cell Growth and Motility. Cancers 2020, 12, 3171. https://doi.org/10.3390/cancers12113171
Wang J-H, Lu T-J, Kung M-L, Yang Y-F, Yeh C-Y, Tu Y-T, Chen W-S, Tsai K-W. The Long Noncoding RNA LOC441461 (STX17-AS1) Modulates Colorectal Cancer Cell Growth and Motility. Cancers. 2020; 12(11):3171. https://doi.org/10.3390/cancers12113171
Chicago/Turabian StyleWang, Jui-Ho, Tzung-Ju Lu, Mei-Lang Kung, Yi-Fang Yang, Chung-Yu Yeh, Ya-Ting Tu, Wei-Shone Chen, and Kuo-Wang Tsai. 2020. "The Long Noncoding RNA LOC441461 (STX17-AS1) Modulates Colorectal Cancer Cell Growth and Motility" Cancers 12, no. 11: 3171. https://doi.org/10.3390/cancers12113171