Abstract
Conventional microfluidic systems that rely on capillary force have a fixed structure and limited sensitivity, which cannot meet the demands of clinical applications. Herein, we propose a dual-drive microfluidic device for sensitive and flexible detection of multiple pathogenic microorganisms antigens/antibodies. The device comprises a portable microfluidic analyzer and a dual-drive microfluidic chip. Along with capillary force, a second active driving force is provided by a removable self-driving valve in the waste chamber. The interval between these two driving forces can be adjusted to control the reaction time in the microchannel, optimizing the formation of antigen-antibody complexes and enhancing sensitivity. Moreover, the material used in the self-driving valve can be changed to adjust the active force strength needed for different tests. The device offers quantitative analysis for respiratory syncytial virus antigen and SARS-CoV-2 antigen using a 35 μL sample, delivering results within 5 min. The detection limits of the system were 1.121 ng/mL and 0.447 ng/mL for respiratory syncytial virus recombinant fusion protein and SARS-CoV-2 recombinant nucleoprotein, respectively. Although the dual-drive microfluidic device has been used for immunoassay for respiratory syncytial virus and SARS-CoV-2 in this study, it can be easily adapted to other immunoassay applications by changing the critical reagents.
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MDPI and ACS Style
Yang, X.; Li, Y.; Lee, J.Z.; Sun, Y.; Tan, X.; Liu, Y.; Yu, Y.; Li, H.; Li, X.
A Highly Sensitive Dual-Drive Microfluidic Device for Multiplexed Detection of Respiratory Virus Antigens. Micromachines 2024, 15, 685.
https://doi.org/10.3390/mi15060685
AMA Style
Yang X, Li Y, Lee JZ, Sun Y, Tan X, Liu Y, Yu Y, Li H, Li X.
A Highly Sensitive Dual-Drive Microfluidic Device for Multiplexed Detection of Respiratory Virus Antigens. Micromachines. 2024; 15(6):685.
https://doi.org/10.3390/mi15060685
Chicago/Turabian Style
Yang, Xiaohui, Yixian Li, Josh Zixi Lee, Yuanmin Sun, Xin Tan, Yijie Liu, Yang Yu, Huiqiang Li, and Xue Li.
2024. "A Highly Sensitive Dual-Drive Microfluidic Device for Multiplexed Detection of Respiratory Virus Antigens" Micromachines 15, no. 6: 685.
https://doi.org/10.3390/mi15060685
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