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Article

Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel

1
Technologies for Precision Medicine, Siemens Healthcare GmbH, Günther-Scharowsky-Str. 1, 91058 Erlangen, Germany
2
Department of Physics and Center for Nanoscience, LMU Munich, Amalienstr. 54, 80799 Munich, Germany
3
Product Lifecycle Management, Siemens Healthcare GmbH, Röntgenstr. 19-21, 95478 Kemnath, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Muhsincan Sesen
Micromachines 2021, 12(4), 358; https://doi.org/10.3390/mi12040358
Received: 27 February 2021 / Revised: 17 March 2021 / Accepted: 22 March 2021 / Published: 26 March 2021
(This article belongs to the Special Issue Microfluidics for Cell Detection and Sorting)
We demonstrate a blood analysis routine by observing red blood cells through light and digital holographic microscopy in a microfluidic channel. With this setup a determination of red blood cell (RBC) concentration, the mean corpuscular volume (MCV), and corpuscular hemoglobin concentration mean (CHCM) is feasible. Cell count variations in between measurements differed by 2.47% with a deviation of 0.26×106 μL to the reference value obtained from the Siemens ADVIA 2120i. Measured MCV values varied by 2.25% and CHCM values by 3.78% compared to the reference ADVIA measurement. Our results suggest that the combination of optical analysis with microfluidics handling provides a promising new approach to red blood cell counts. View Full-Text
Keywords: blood analysis; RBC counting; cellular hemoglobin concentration; cellular volume; microfluidics; hydrodynamic focusing; digital holographic microscopy blood analysis; RBC counting; cellular hemoglobin concentration; cellular volume; microfluidics; hydrodynamic focusing; digital holographic microscopy
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MDPI and ACS Style

Reichenwallner, A.-K.; Vurmaz, E.; Battis, K.; Handl, L.; Üstün, H.; Mach, T.; Hörnig, G.; Lipfert, J.; Richter, L. Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel. Micromachines 2021, 12, 358. https://doi.org/10.3390/mi12040358

AMA Style

Reichenwallner A-K, Vurmaz E, Battis K, Handl L, Üstün H, Mach T, Hörnig G, Lipfert J, Richter L. Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel. Micromachines. 2021; 12(4):358. https://doi.org/10.3390/mi12040358

Chicago/Turabian Style

Reichenwallner, Ann-Kathrin, Esma Vurmaz, Kristina Battis, Laura Handl, Helin Üstün, Tivadar Mach, Gabriele Hörnig, Jan Lipfert, and Lukas Richter. 2021. "Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel" Micromachines 12, no. 4: 358. https://doi.org/10.3390/mi12040358

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