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Open AccessArticle

Optical Projection Tomography Using a Commercial Microfluidic System

1
Center for Optics Research and Engineering, Shandong University, Qingdao 266237, China
2
School of Information Science and Engineering, Shandong University, Qingdao 266237, China
3
Key Laboratory of Infrared Imaging Materials and Devices, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(3), 293; https://doi.org/10.3390/mi11030293
Received: 16 February 2020 / Revised: 5 March 2020 / Accepted: 10 March 2020 / Published: 11 March 2020
(This article belongs to the Special Issue Nano-Bio Optical Devices and Applications)
Optical projection tomography (OPT) is the direct optical equivalent of X-ray computed tomography (CT). To obtain a larger depth of field, traditional OPT usually decreases the numerical aperture (NA) of the objective lens to decrease the resolution of the image. So, there is a trade-off between sample size and resolution. Commercial microfluidic systems can observe a sample in flow mode. In this paper, an OPT instrument is constructed to observe samples. The OPT instrument is combined with commercial microfluidic systems to obtain a three-dimensional and time (3D + T)/four-dimensional (4D) video of the sample. “Focal plane scanning” is also used to increase the images’ depth of field. A series of two-dimensional (2D) images in different focal planes was observed and compared with images simulated using our program. Our work dynamically monitors 3D OPT images. Commercial microfluidic systems simulate blood flow, which has potential application in blood monitoring and intelligent drug delivery platforms. We design an OPT adaptor to perform OPT on a commercial wide-field inverted microscope (Olympusix81). Images in different focal planes are observed and analyzed. Using a commercial microfluidic system, a video is also acquired to record motion pictures of samples at different flow rates. To our knowledge, this is the first time an OPT setup has been combined with a microfluidic system. View Full-Text
Keywords: microfluidics; OPT; microscope; lab-on-a-chip; focal plane scanning microfluidics; OPT; microscope; lab-on-a-chip; focal plane scanning
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MDPI and ACS Style

Du, W.; Fei, C.; Liu, J.; Li, Y.; Liu, Z.; Zhao, X.; Fang, J. Optical Projection Tomography Using a Commercial Microfluidic System. Micromachines 2020, 11, 293.

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