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Open AccessArticle

Simultaneous Determination of Four DNA bases at Graphene Oxide/Multi-Walled Carbon Nanotube Nanocomposite-Modified Electrode

by Shuting Wang 1,†, Celia Ferrag 1,2,†, Meissam Noroozifar 1 and Kagan Kerman 1,2,*
1
Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada
2
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada
*
Author to whom correspondence should be addressed.
These authors contributed equally to the work.
Micromachines 2020, 11(3), 294; https://doi.org/10.3390/mi11030294
Received: 17 February 2020 / Revised: 5 March 2020 / Accepted: 9 March 2020 / Published: 11 March 2020
(This article belongs to the Special Issue Graphene Nanoelectronic Devices)
In this study, we developed a modified glassy carbon electrode (GCE) with graphene oxide, multi-walled carbon nanotube hybrid nanocomposite in chitosan (GCE/GO-MWCNT-CHT) to achieve simultaneous detection of four nucleobases (i.e., guanine (G), adenine (A), thymine (T) and cytosine (C)) along with uric acid (UA) as an internal standard. The nanocomposite was characterized using TEM and FT-IR. The linearity ranges were up to 151.0, 78.0, 79.5, 227.5, and 162.5 µM with a detection limit of 0.15, 0.12, 0.44, 4.02, 4.0, and 3.30 µM for UA, G, A, T, and C, respectively. Compared to a bare GCE, the nanocomposite-modified GCE demonstrated a large enhancement (~36.6%) of the electrochemical active surface area. Through chronoamperometric studies, the diffusion coefficients (D), standard catalytic rate constant (Ks), and heterogenous rate constant (Kh) were calculated for the analytes. Moreover, the nanocomposite-modified electrode was used for simultaneous detection in human serum, human saliva, and artificial saliva samples with recovery values ranging from 95% to 105%. View Full-Text
Keywords: graphene oxide; multi-walled carbon nanotube; nanocomposite; guanine; adenine; cytosine; thymine; uric acid; voltammetry graphene oxide; multi-walled carbon nanotube; nanocomposite; guanine; adenine; cytosine; thymine; uric acid; voltammetry
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MDPI and ACS Style

Wang, S.; Ferrag, C.; Noroozifar, M.; Kerman, K. Simultaneous Determination of Four DNA bases at Graphene Oxide/Multi-Walled Carbon Nanotube Nanocomposite-Modified Electrode. Micromachines 2020, 11, 294.

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