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Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization

1
INSERM UMR-S1147, CNRS SNC5014, Paris Descartes University, Equipe Labellisée Ligue Nationale Contre le Cancer, 75005 Paris, France
2
Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Ferrara, 44121 Ferrara, Italy
*
Authors to whom correspondence should be addressed.
Present address: Département de Chimie, UMR 8640 PASTEUR, Ecole Normale Supérieure-PSL Research University, 75005 Paris, France.
Micromachines 2019, 10(4), 265; https://doi.org/10.3390/mi10040265
Received: 19 March 2019 / Revised: 2 April 2019 / Accepted: 9 April 2019 / Published: 19 April 2019
(This article belongs to the Special Issue Micro/Nano-system for Drug Delivery)
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Abstract

Traditional two-dimensional (2D) cell culture models are limited in their ability to reproduce human structures and functions. On the contrary, three-dimensional (3D) microtissues have the potential to permit the development of new cell-based assays as advanced in vitro models to test new drugs. Here, we report the use of a dehydrated gelatin film to promote tumor cells aggregation and 3D microtissue formation. The simple and stable gelatin coating represents an alternative to conventional and expensive materials like type I collagen, hyaluronic acid, or matrigel. The gelatin coating is biocompatible with several culture formats including microfluidic chips, as well as standard micro-well plates. It also enables long-term 3D cell culture and in situ monitoring of live/dead assays. View Full-Text
Keywords: 3D microtissues; microfluidics; human colon adenocarcinoma; cells viability; dehydration; gelatin; hydrogel 3D microtissues; microfluidics; human colon adenocarcinoma; cells viability; dehydration; gelatin; hydrogel
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Pitingolo, G.; Riaud, A.; Nastruzzi, C.; Taly, V. Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization. Micromachines 2019, 10, 265.

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