Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials and Equipment
2.2. Fabrication of Microfluidic Chips and Gelatin Coating
2.3. Cell Seeding and Characterization
3. Results and Discussions
3.1. Fabrication of Gelatin-Coated Microfluidic Channels
3.2. Cells Aggregation and 3D Microtissue Formation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Day | Semi-Major Axis Coated (µm) | Semi-Minor Axis Coated (µm) | Semi-Major Axis Uncoated (µm) | Semi-Minor Axis Uncoated (µm) | Total Area Coated (mm2) | Total Area Uncoated (mm2) |
---|---|---|---|---|---|---|
7 | 201 ± 20 | 153 ± 12 | 50 ± 10 | 41 ± 7 | 0.965 | 0.064 |
8 | 216 ± 24 | 162 ± 18 | 53 ± 8 | 43 ± 4 | 1.098 | 0.071 |
9 | 249 ± 18 | 165 ± 26 | 60 ± 12 | 47 ± 9 | 1.290 | 0.088 |
10 | 262 ± 24 | 172 ± 9 | 72 ± 9 | 54 ± 11 | 1.415 | 0.122 |
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Pitingolo, G.; Riaud, A.; Nastruzzi, C.; Taly, V. Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization. Micromachines 2019, 10, 265. https://doi.org/10.3390/mi10040265
Pitingolo G, Riaud A, Nastruzzi C, Taly V. Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization. Micromachines. 2019; 10(4):265. https://doi.org/10.3390/mi10040265
Chicago/Turabian StylePitingolo, Gabriele, Antoine Riaud, Claudio Nastruzzi, and Valerie Taly. 2019. "Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization" Micromachines 10, no. 4: 265. https://doi.org/10.3390/mi10040265
APA StylePitingolo, G., Riaud, A., Nastruzzi, C., & Taly, V. (2019). Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization. Micromachines, 10(4), 265. https://doi.org/10.3390/mi10040265