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Open AccessArticle

A New Member of Gamma-Conotoxin Family Isolated from Conus princeps Displays a Novel Molecular Target

1
Departamento de Innovación Biomédica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, Baja California, C.P. 22860, Mexico
2
Laboratorio de Espectrometría de Masas, Departamento de Proteómica, Centro de Ingeniería Genética y Biotecnología, Avenida 31 e/158 y 190, Cubanacán, Playa, PO Box 6162. C.P. 10600, La Habana, Cuba
3
Instituto de Fisiología, Benemerita Universidad de Puebla, 14 sur 6301, CU, San Manuel, Puebla, Pue, C.P. 72570, Mexico
4
Laboratorio de Neurofarmacología Marina, Departamento de Neurobiología Celular y Molecular, UNAM, Juriquilla, Queretaro, C.P. 76230, Mexico
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Luis M. Botana
Toxins 2016, 8(2), 39; https://doi.org/10.3390/toxins8020039
Received: 3 November 2015 / Revised: 22 January 2016 / Accepted: 25 January 2016 / Published: 5 February 2016
(This article belongs to the Collection Marine and Freshwater Toxins)
A novel conotoxin, named as PiVIIA, was isolated from the venom of Conus princeps, a marine predatory cone snail collected in the Pacific Southern Coast of Mexico. Chymotryptic digest of the S-alkylated peptide in combination with liquid chromatography coupled to tandem mass spectrometry, were used to define the sequencing of this peptide. Eleven N-terminal amino acids were verified by automated Edman degradation. PiVIIA is a 25-mer peptide (CDAOTHYCTNYWγCCSGYCγHSHCW) with six cysteine residues forming three disulphide bonds, a hydroxyproline (O) and two gamma carboxyglutamic acid (γ) residues. Based on the arrangement of six Cys residues (C-C-CC-C-C), this conotoxin might belong to the O2-superfamily. Moreover, PiVIIA has a conserved motif (-γCCS-) that characterizes γ-conotoxins from molluscivorous Conus. Peptide PiVIIA has 45% sequence identity with γ-PnVIIA—the prototype of this family. Biological activity of PiVIIA was assessed by voltage-clamp recording in rat dorsal root ganglion neurons. Perfusion of PiVIIA in the µM range produces a significant increase in the Ca2+ currents, without significantly modifying the Na+, K+ or proton-gated acid sensing ionic currents. These results indicate that PiVIIA is a new conotoxin whose activity deserves further studies to define its potential use as a positive modulator of neuronal activity. View Full-Text
Keywords: Conus princeps; calcium channel; gamma-conotoxin; mass spectrometry; dorsal root ganglion neurons Conus princeps; calcium channel; gamma-conotoxin; mass spectrometry; dorsal root ganglion neurons
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MDPI and ACS Style

Bernáldez, J.; Jiménez, S.; González, L.J.; Ferro, J.N.; Soto, E.; Salceda, E.; Chávez, D.; Aguilar, M.B.; Licea-Navarro, A. A New Member of Gamma-Conotoxin Family Isolated from Conus princeps Displays a Novel Molecular Target. Toxins 2016, 8, 39.

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