A New Member of Gamma-Conotoxin Family Isolated from Conus princeps Displays a Novel Molecular Target
AbstractA novel conotoxin, named as PiVIIA, was isolated from the venom of Conus princeps, a marine predatory cone snail collected in the Pacific Southern Coast of Mexico. Chymotryptic digest of the S-alkylated peptide in combination with liquid chromatography coupled to tandem mass spectrometry, were used to define the sequencing of this peptide. Eleven N-terminal amino acids were verified by automated Edman degradation. PiVIIA is a 25-mer peptide (CDAOTHYCTNYWγCCSGYCγHSHCW) with six cysteine residues forming three disulphide bonds, a hydroxyproline (O) and two gamma carboxyglutamic acid (γ) residues. Based on the arrangement of six Cys residues (C-C-CC-C-C), this conotoxin might belong to the O2-superfamily. Moreover, PiVIIA has a conserved motif (-γCCS-) that characterizes γ-conotoxins from molluscivorous Conus. Peptide PiVIIA has 45% sequence identity with γ-PnVIIA—the prototype of this family. Biological activity of PiVIIA was assessed by voltage-clamp recording in rat dorsal root ganglion neurons. Perfusion of PiVIIA in the µM range produces a significant increase in the Ca2+ currents, without significantly modifying the Na+, K+ or proton-gated acid sensing ionic currents. These results indicate that PiVIIA is a new conotoxin whose activity deserves further studies to define its potential use as a positive modulator of neuronal activity. View Full-Text
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Bernáldez, J.; Jiménez, S.; González, L.J.; Ferro, J.N.; Soto, E.; Salceda, E.; Chávez, D.; Aguilar, M.B.; Licea-Navarro, A. A New Member of Gamma-Conotoxin Family Isolated from Conus princeps Displays a Novel Molecular Target. Toxins 2016, 8, 39.
Bernáldez J, Jiménez S, González LJ, Ferro JN, Soto E, Salceda E, Chávez D, Aguilar MB, Licea-Navarro A. A New Member of Gamma-Conotoxin Family Isolated from Conus princeps Displays a Novel Molecular Target. Toxins. 2016; 8(2):39.Chicago/Turabian Style
Bernáldez, Johanna; Jiménez, Samanta; González, Luis J.; Ferro, Jesús N.; Soto, Enrique; Salceda, Emilio; Chávez, Daniela; Aguilar, Manuel B.; Licea-Navarro, Alexei. 2016. "A New Member of Gamma-Conotoxin Family Isolated from Conus princeps Displays a Novel Molecular Target." Toxins 8, no. 2: 39.
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